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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M49 Chaperone protein DnaK (dnaK), partial CSB-YP475346FNH
CSB-EP475346FNH
CSB-BP475346FNH
CSB-MP475346FNH
CSB-EP475346FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Protein translocase subunit SecA (secA), partial CSB-YP475347FNH
CSB-EP475347FNH
CSB-BP475347FNH
CSB-MP475347FNH
CSB-EP475347FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribonuclease HIII (rnhC) CSB-YP475348FNH
CSB-EP475348FNH
CSB-BP475348FNH
CSB-MP475348FNH
CSB-EP475348FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Phosphoglycerate kinase (pgk) CSB-YP475349FNH
CSB-EP475349FNH
CSB-BP475349FNH
CSB-MP475349FNH
CSB-EP475349FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Proline--tRNA ligase (proS), partial CSB-YP475350FNH
CSB-EP475350FNH
CSB-BP475350FNH
CSB-MP475350FNH
CSB-EP475350FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Imidazolonepropionase (hutI) CSB-YP475351FNH
CSB-EP475351FNH
CSB-BP475351FNH
CSB-MP475351FNH
CSB-EP475351FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 DNA repair protein recO (recO) CSB-YP475352FNH
CSB-EP475352FNH
CSB-BP475352FNH
CSB-MP475352FNH
CSB-EP475352FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L16 (rplP) CSB-YP475353FNH
CSB-EP475353FNH
CSB-BP475353FNH
CSB-MP475353FNH
CSB-EP475353FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L24 (rplX) CSB-YP475354FNH
CSB-EP475354FNH
CSB-BP475354FNH
CSB-MP475354FNH
CSB-EP475354FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L15 (rplO) CSB-YP475355FNH
CSB-EP475355FNH
CSB-BP475355FNH
CSB-MP475355FNH
CSB-EP475355FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Tyrosine--tRNA ligase (tyrS) CSB-YP475356FNH
CSB-EP475356FNH
CSB-BP475356FNH
CSB-MP475356FNH
CSB-EP475356FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP475357FNH
CSB-EP475357FNH
CSB-BP475357FNH
CSB-MP475357FNH
CSB-EP475357FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Acetate kinase (ackA) CSB-YP475358FNH
CSB-EP475358FNH
CSB-BP475358FNH
CSB-MP475358FNH
CSB-EP475358FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Leucine--tRNA ligase (leuS), partial CSB-YP475359FNH
CSB-EP475359FNH
CSB-BP475359FNH
CSB-MP475359FNH
CSB-EP475359FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribonuclease P protein component (rnpA) CSB-YP475360FNH
CSB-EP475360FNH
CSB-BP475360FNH
CSB-MP475360FNH
CSB-EP475360FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0348 protein Spy49_0264 (Spy49_0264) CSB-YP475361FNH
CSB-EP475361FNH
CSB-BP475361FNH
CSB-MP475361FNH
CSB-EP475361FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Segregation and condensation protein A (scpA) CSB-YP475362FNH
CSB-EP475362FNH
CSB-BP475362FNH
CSB-MP475362FNH
CSB-EP475362FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L1 (rplA) CSB-YP475363FNH
CSB-EP475363FNH
CSB-BP475363FNH
CSB-MP475363FNH
CSB-EP475363FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Lysine--tRNA ligase (lysS) CSB-YP475364FNH
CSB-EP475364FNH
CSB-BP475364FNH
CSB-MP475364FNH
CSB-EP475364FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L31 type B (rpmE2) CSB-YP475365FNH
CSB-EP475365FNH
CSB-BP475365FNH
CSB-MP475365FNH
CSB-EP475365FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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