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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella heidelberg Protein slyX (slyX) CSB-YP474566SWQ
CSB-EP474566SWQ
CSB-BP474566SWQ
CSB-MP474566SWQ
CSB-EP474566SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Shikimate kinase 1 (aroK) CSB-YP474567SWQ
CSB-EP474567SWQ
CSB-BP474567SWQ
CSB-MP474567SWQ
CSB-EP474567SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Fe/S biogenesis protein NfuA (nfuA) CSB-YP474568SWQ
CSB-EP474568SWQ
CSB-BP474568SWQ
CSB-MP474568SWQ
CSB-EP474568SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP474569SWV
CSB-EP474569SWV
CSB-BP474569SWV
CSB-MP474569SWV
CSB-EP474569SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP474570SWV
CSB-EP474570SWV
CSB-BP474570SWV
CSB-MP474570SWV
CSB-EP474570SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribonuclease P protein component (rnpA) CSB-YP474571SWV
CSB-EP474571SWV
CSB-BP474571SWV
CSB-MP474571SWV
CSB-EP474571SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-ribose pyranase (rbsD) CSB-YP474572SWV
CSB-EP474572SWV
CSB-BP474572SWV
CSB-MP474572SWV
CSB-EP474572SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Multidrug resistance protein MdtC (mdtC), partial CSB-YP474573SWV
CSB-EP474573SWV
CSB-BP474573SWV
CSB-MP474573SWV
CSB-EP474573SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 4-alpha-L-fucosyltransferase (wecF) CSB-YP474574SWV
CSB-EP474574SWV
CSB-BP474574SWV
CSB-MP474574SWV
CSB-EP474574SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP474575SWV1
CSB-EP474575SWV1
CSB-BP474575SWV1
CSB-MP474575SWV1
CSB-EP474575SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L25 (rplY) CSB-YP474576SWV
CSB-EP474576SWV
CSB-BP474576SWV
CSB-MP474576SWV
CSB-EP474576SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Lipopolysaccharide core heptose (II)-phosphate phosphatase CSB-YP474577SWV
CSB-EP474577SWV
CSB-BP474577SWV
CSB-MP474577SWV
CSB-EP474577SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP474578SWV1
CSB-EP474578SWV1
CSB-BP474578SWV1
CSB-MP474578SWV1
CSB-EP474578SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP474579SWV
CSB-EP474579SWV
CSB-BP474579SWV
CSB-MP474579SWV
CSB-EP474579SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund L-rhamnose mutarotase (rhaM) CSB-YP474580SWV
CSB-EP474580SWV
CSB-BP474580SWV
CSB-MP474580SWV
CSB-EP474580SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund HTH-type transcriptional activator rhaS (rhaS) CSB-YP474581SWV
CSB-EP474581SWV
CSB-BP474581SWV
CSB-MP474581SWV
CSB-EP474581SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP474582SWV
CSB-EP474582SWV
CSB-BP474582SWV
CSB-MP474582SWV
CSB-EP474582SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) CSB-YP474583SWV
CSB-EP474583SWV
CSB-BP474583SWV
CSB-MP474583SWV
CSB-EP474583SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Potassium-transporting ATPase A chain (kdpA), partial CSB-YP474584SWV
CSB-EP474584SWV
CSB-BP474584SWV
CSB-MP474584SWV
CSB-EP474584SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Penicillin-insensitive murein endopeptidase (mepA) CSB-YP474585SWV
CSB-EP474585SWV
CSB-BP474585SWV
CSB-MP474585SWV
CSB-EP474585SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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