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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii UvrABC system protein B (uvrB), partial CSB-YP475966DXN
CSB-EP475966DXN
CSB-BP475966DXN
CSB-MP475966DXN
CSB-EP475966DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Adenylate kinase (adk) CSB-YP475967DXN
CSB-EP475967DXN
CSB-BP475967DXN
CSB-MP475967DXN
CSB-EP475967DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP475968DXN
CSB-EP475968DXN
CSB-BP475968DXN
CSB-MP475968DXN
CSB-EP475968DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Trigger factor (tig) CSB-YP475969DXN
CSB-EP475969DXN
CSB-BP475969DXN
CSB-MP475969DXN
CSB-EP475969DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Elongation factor P (efp) CSB-YP475970DXN
CSB-EP475970DXN
CSB-BP475970DXN
CSB-MP475970DXN
CSB-EP475970DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Leucine--tRNA ligase (leuS), partial CSB-YP475971OED
CSB-EP475971OED
CSB-BP475971OED
CSB-MP475971OED
CSB-EP475971OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Probable intracellular septation protein A (OCAR_4259, OCA5_c02590), partial CSB-YP475972OED1
CSB-EP475972OED1
CSB-BP475972OED1
CSB-MP475972OED1
CSB-EP475972OED1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP475973OED
CSB-EP475973OED
CSB-BP475973OED
CSB-MP475973OED
CSB-EP475973OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans UPF0235 protein OCAR_4310/OCA5_c02140 (OCAR_4310, OCA5_c02140) CSB-YP475974OED
CSB-EP475974OED
CSB-BP475974OED
CSB-MP475974OED
CSB-EP475974OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP475975OED
CSB-EP475975OED
CSB-BP475975OED
CSB-MP475975OED
CSB-EP475975OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Ribonuclease PH (rph) CSB-YP475976OED
CSB-EP475976OED
CSB-BP475976OED
CSB-MP475976OED
CSB-EP475976OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP475977OED
CSB-EP475977OED
CSB-BP475977OED
CSB-MP475977OED
CSB-EP475977OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Undecaprenyl-diphosphatase (uppP), partial CSB-YP475978OED1
CSB-EP475978OED1
CSB-BP475978OED1
CSB-MP475978OED1
CSB-EP475978OED1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP475979OED
CSB-EP475979OED
CSB-BP475979OED
CSB-MP475979OED
CSB-EP475979OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S15 (rpsO) CSB-YP475980OED
CSB-EP475980OED
CSB-BP475980OED
CSB-MP475980OED
CSB-EP475980OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Glutamate 5-kinase (proB) CSB-YP475981OED
CSB-EP475981OED
CSB-BP475981OED
CSB-MP475981OED
CSB-EP475981OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Protein hfq (hfq) CSB-YP475982OED
CSB-EP475982OED
CSB-BP475982OED
CSB-MP475982OED
CSB-EP475982OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L1 (rplA) CSB-YP475983OED
CSB-EP475983OED
CSB-BP475983OED
CSB-MP475983OED
CSB-EP475983OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L16 (rplP) CSB-YP475984OED
CSB-EP475984OED
CSB-BP475984OED
CSB-MP475984OED
CSB-EP475984OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S8 (rpsH) CSB-YP475985OED
CSB-EP475985OED
CSB-BP475985OED
CSB-MP475985OED
CSB-EP475985OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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