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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella heidelberg tRNA sulfurtransferase (thiI) CSB-YP474486SWQ
CSB-EP474486SWQ
CSB-BP474486SWQ
CSB-MP474486SWQ
CSB-EP474486SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0265 protein yeeX (yeeX) CSB-YP474487SWQ
CSB-EP474487SWQ
CSB-BP474487SWQ
CSB-MP474487SWQ
CSB-EP474487SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg L-seryl-tRNA (Sec) selenium transferase CSB-YP474488SWQ
CSB-EP474488SWQ
CSB-BP474488SWQ
CSB-MP474488SWQ
CSB-EP474488SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP474489SWQ
CSB-EP474489SWQ
CSB-BP474489SWQ
CSB-MP474489SWQ
CSB-EP474489SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0234 protein yajQ (yajQ) CSB-YP474490SWQ
CSB-EP474490SWQ
CSB-BP474490SWQ
CSB-MP474490SWQ
CSB-EP474490SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ureidoglycolate hydrolase (allA) CSB-YP474491SWQ
CSB-EP474491SWQ
CSB-BP474491SWQ
CSB-MP474491SWQ
CSB-EP474491SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Deoxycytidine triphosphate deaminase (dcd) CSB-YP474492SWQ
CSB-EP474492SWQ
CSB-BP474492SWQ
CSB-MP474492SWQ
CSB-EP474492SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Orotate phosphoribosyltransferase (pyrE) CSB-YP474493SWQ
CSB-EP474493SWQ
CSB-BP474493SWQ
CSB-MP474493SWQ
CSB-EP474493SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Small heat shock protein ibpB (ibpB) CSB-YP474494SWQ
CSB-EP474494SWQ
CSB-BP474494SWQ
CSB-MP474494SWQ
CSB-EP474494SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable endonuclease 4 (nfo) CSB-YP474495SWQ
CSB-EP474495SWQ
CSB-BP474495SWQ
CSB-MP474495SWQ
CSB-EP474495SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Periplasmic nitrate reductase (napA), partial CSB-YP474496SWQ
CSB-EP474496SWQ
CSB-BP474496SWQ
CSB-MP474496SWQ
CSB-EP474496SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Bifunctional protein GlmU (glmU) CSB-YP474497SWQ
CSB-EP474497SWQ
CSB-BP474497SWQ
CSB-MP474497SWQ
CSB-EP474497SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg ATP synthase subunit c (atpE), partial CSB-YP474498SWQ1
CSB-EP474498SWQ1
CSB-BP474498SWQ1
CSB-MP474498SWQ1
CSB-EP474498SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg HTH-type transcriptional regulator hdfR (hdfR) CSB-YP474499SWQ
CSB-EP474499SWQ
CSB-BP474499SWQ
CSB-MP474499SWQ
CSB-EP474499SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP474500SWQ
CSB-EP474500SWQ
CSB-BP474500SWQ
CSB-MP474500SWQ
CSB-EP474500SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable rRNA maturation factor YbeY (ybeY) CSB-YP474501SWQ
CSB-EP474501SWQ
CSB-BP474501SWQ
CSB-MP474501SWQ
CSB-EP474501SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP474502SWQ
CSB-EP474502SWQ
CSB-BP474502SWQ
CSB-MP474502SWQ
CSB-EP474502SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP474503SWQ1
CSB-EP474503SWQ1
CSB-BP474503SWQ1
CSB-MP474503SWQ1
CSB-EP474503SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP474504SWQ
CSB-EP474504SWQ
CSB-BP474504SWQ
CSB-MP474504SWQ
CSB-EP474504SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP474505SWQ1
CSB-EP474505SWQ1
CSB-BP474505SWQ1
CSB-MP474505SWQ1
CSB-EP474505SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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