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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP474626SWV
CSB-EP474626SWV
CSB-BP474626SWV
CSB-MP474626SWV
CSB-EP474626SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP474627SWV
CSB-EP474627SWV
CSB-BP474627SWV
CSB-MP474627SWV
CSB-EP474627SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Tryptophan synthase beta chain (trpB) CSB-YP474628SWV
CSB-EP474628SWV
CSB-BP474628SWV
CSB-MP474628SWV
CSB-EP474628SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein AaeX (aaeX), partial CSB-YP474629SWV1
CSB-EP474629SWV1
CSB-BP474629SWV1
CSB-MP474629SWV1
CSB-EP474629SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S8 (rpsH) CSB-YP474630SWV
CSB-EP474630SWV
CSB-BP474630SWV
CSB-MP474630SWV
CSB-EP474630SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S3 (rpsC) CSB-YP474631SWV
CSB-EP474631SWV
CSB-BP474631SWV
CSB-MP474631SWV
CSB-EP474631SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP474632SWV
CSB-EP474632SWV
CSB-BP474632SWV
CSB-MP474632SWV
CSB-EP474632SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund GMP reductase (guaC) CSB-YP474633SWV
CSB-EP474633SWV
CSB-BP474633SWV
CSB-MP474633SWV
CSB-EP474633SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Sugar fermentation stimulation protein A (sfsA) CSB-YP474634SWV
CSB-EP474634SWV
CSB-BP474634SWV
CSB-MP474634SWV
CSB-EP474634SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP474635SWV
CSB-EP474635SWV
CSB-BP474635SWV
CSB-MP474635SWV
CSB-EP474635SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Fatty acid metabolism regulator protein (fadR) CSB-YP474636SWV
CSB-EP474636SWV
CSB-BP474636SWV
CSB-MP474636SWV
CSB-EP474636SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund ProP effector (proQ) CSB-YP474637SWV
CSB-EP474637SWV
CSB-BP474637SWV
CSB-MP474637SWV
CSB-EP474637SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Shikimate kinase 1 (aroK) CSB-YP474638SWV
CSB-EP474638SWV
CSB-BP474638SWV
CSB-MP474638SWV
CSB-EP474638SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Proline--tRNA ligase (proS), partial CSB-YP474639SWV
CSB-EP474639SWV
CSB-BP474639SWV
CSB-MP474639SWV
CSB-EP474639SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein yebF (yebF) CSB-YP474640SWV
CSB-EP474640SWV
CSB-BP474640SWV
CSB-MP474640SWV
CSB-EP474640SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund tRNA (cmo5U34)-methyltransferase CSB-YP474641SWV
CSB-EP474641SWV
CSB-BP474641SWV
CSB-MP474641SWV
CSB-EP474641SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP474642SWV
CSB-EP474642SWV
CSB-BP474642SWV
CSB-MP474642SWV
CSB-EP474642SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 2,3-diketo-L-gulonate reductase (dlgD) CSB-YP474643SWV
CSB-EP474643SWV
CSB-BP474643SWV
CSB-MP474643SWV
CSB-EP474643SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Shikimate kinase 2 (aroL) CSB-YP474644SWV
CSB-EP474644SWV
CSB-BP474644SWV
CSB-MP474644SWV
CSB-EP474644SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cobalamin synthase (cobS), partial CSB-YP474645SWV1
CSB-EP474645SWV1
CSB-BP474645SWV1
CSB-MP474645SWV1
CSB-EP474645SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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