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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP475566EOE
CSB-EP475566EOE
CSB-BP475566EOE
CSB-MP475566EOE
CSB-EP475566EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP475567EOE
CSB-EP475567EOE
CSB-BP475567EOE
CSB-MP475567EOE
CSB-EP475567EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP475568EOE
CSB-EP475568EOE
CSB-BP475568EOE
CSB-MP475568EOE
CSB-EP475568EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L25 (rplY) CSB-YP475569EOE
CSB-EP475569EOE
CSB-BP475569EOE
CSB-MP475569EOE
CSB-EP475569EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP475570EOE
CSB-EP475570EOE
CSB-BP475570EOE
CSB-MP475570EOE
CSB-EP475570EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA replication and repair protein recF (recF) CSB-YP475571EOE
CSB-EP475571EOE
CSB-BP475571EOE
CSB-MP475571EOE
CSB-EP475571EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional regulator yidZ (yidZ) CSB-YP475572EOE
CSB-EP475572EOE
CSB-BP475572EOE
CSB-MP475572EOE
CSB-EP475572EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cell division topological specificity factor (minE) CSB-YP475573EOE
CSB-EP475573EOE
CSB-BP475573EOE
CSB-MP475573EOE
CSB-EP475573EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Peptidyl-tRNA hydrolase (pth) CSB-YP475574EOE
CSB-EP475574EOE
CSB-BP475574EOE
CSB-MP475574EOE
CSB-EP475574EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP475575EOE
CSB-EP475575EOE
CSB-BP475575EOE
CSB-MP475575EOE
CSB-EP475575EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP475576EOE1
CSB-EP475576EOE1
CSB-BP475576EOE1
CSB-MP475576EOE1
CSB-EP475576EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP475577EOE
CSB-EP475577EOE
CSB-BP475577EOE
CSB-MP475577EOE
CSB-EP475577EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Fatty acid oxidation complex subunit alpha (fadJ) CSB-YP475578EOE
CSB-EP475578EOE
CSB-BP475578EOE
CSB-MP475578EOE
CSB-EP475578EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Low affinity potassium transport system protein kup (kup), partial CSB-YP475579EOE
CSB-EP475579EOE
CSB-BP475579EOE
CSB-MP475579EOE
CSB-EP475579EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putative ECA polymerase (wzyE) CSB-YP475580EOE
CSB-EP475580EOE
CSB-BP475580EOE
CSB-MP475580EOE
CSB-EP475580EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP475581EOE1
CSB-EP475581EOE1
CSB-BP475581EOE1
CSB-MP475581EOE1
CSB-EP475581EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Carnitine operon protein CaiE (caiE) CSB-YP475582EOE
CSB-EP475582EOE
CSB-BP475582EOE
CSB-MP475582EOE
CSB-EP475582EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Formyl-coenzyme A transferase (frc) CSB-YP475583EOE
CSB-EP475583EOE
CSB-BP475583EOE
CSB-MP475583EOE
CSB-EP475583EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein FdhE (fdhE) CSB-YP475584EOE
CSB-EP475584EOE
CSB-BP475584EOE
CSB-MP475584EOE
CSB-EP475584EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 L-rhamnose isomerase (rhaA) CSB-YP475585EOE
CSB-EP475585EOE
CSB-BP475585EOE
CSB-MP475585EOE
CSB-EP475585EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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