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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Retinal dehydrogenase 1 (ALDH1A1) CSB-YP001565HU
CSB-EP001565HU
CSB-BP001565HU
CSB-MP001565HU
CSB-EP001565HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Glutamate dehydrogenase 1, mitochondrial (GLUD1), partial CSB-YP009544BO
CSB-EP009544BO
CSB-BP009544BO
CSB-MP009544BO
CSB-EP009544BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig D-amino-acid oxidase (DAO) CSB-YP006494PI
CSB-EP006494PI
CSB-BP006494PI
CSB-MP006494PI
CSB-EP006494PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Dihydrofolate reductase (DHFR) CSB-YP006847CH
CSB-EP006847CH
CSB-BP006847CH
CSB-MP006847CH
CSB-EP006847CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit NADPH--cytochrome P450 reductase (POR), partial CSB-YP018378RB
CSB-EP018378RB
CSB-BP018378RB
CSB-MP018378RB
CSB-EP018378RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073HU1
CSB-EP015073HU1
CSB-BP015073HU1
CSB-MP015073HU1
CSB-EP015073HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome c oxidase subunit 5B, mitochondrial (COX5B) CSB-YP005839BO
CSB-EP005839BO
CSB-BP005839BO
CSB-MP005839BO
CSB-EP005839BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Glutathione peroxidase 1 (GPX1) CSB-YP009866BO
CSB-EP009866BO
CSB-BP009866BO
CSB-MP009866BO
CSB-EP009866BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phenylalanine-4-hydroxylase (PAH) CSB-BP017396HU
CSB-MP017396HU
CSB-EP017396HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Glycogen phosphorylase, muscle form (PYGM), partial CSB-YP019123RB
CSB-EP019123RB
CSB-BP019123RB
CSB-MP019123RB
CSB-EP019123RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Hypoxanthine-guanine phosphoribosyltransferase (Hprt1) CSB-YP010706MO
CSB-EP010706MO
CSB-BP010706MO
CSB-MP010706MO
CSB-EP010706MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Aspartate aminotransferase, mitochondrial (GOT2) CSB-YP009681HU
CSB-BP009681HU
CSB-MP009681HU
CSB-EP009681HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine cGMP-dependent protein kinase 1 (PRKG1), partial CSB-YP018715BO
CSB-EP018715BO
CSB-BP018715BO
CSB-MP018715BO
CSB-EP018715BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tyrosine-protein kinase ABL1 (ABL1), partial CSB-YP001105HU
CSB-EP001105HU
CSB-BP001105HU
CSB-MP001105HU
CSB-EP001105HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Proto-oncogene tyrosine-protein kinase Src (SRC), partial CSB-YP022650CH
CSB-EP022650CH
CSB-BP022650CH
CSB-MP022650CH
CSB-EP022650CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Proto-oncogene serine/threonine-protein kinase mos (MOS) CSB-YP014733HU
CSB-EP014733HU
CSB-BP014733HU
CSB-MP014733HU
CSB-EP014733HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Creatine kinase M-type (CKM) CSB-YP005459CH
CSB-EP005459CH
CSB-BP005459CH
CSB-MP005459CH
CSB-EP005459CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Adenylate kinase isoenzyme 1 (AK1) CSB-YP001508BO
CSB-EP001508BO
CSB-BP001508BO
CSB-MP001508BO
CSB-EP001508BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Pancreatic triacylglycerol lipase (PNLIP) CSB-YP018262PI
CSB-EP018262PI
CSB-BP018262PI
CSB-MP018262PI
CSB-EP018262PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Fructose-1,6-bisphosphatase 1 (FBP1) CSB-YP008459PI
CSB-EP008459PI
CSB-BP008459PI
CSB-MP008459PI
CSB-EP008459PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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