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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella gallinarum tRNA 2-selenouridine synthase (selU) CSB-YP475206SWP
CSB-EP475206SWP
CSB-BP475206SWP
CSB-MP475206SWP
CSB-EP475206SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP475207SWP
CSB-EP475207SWP
CSB-BP475207SWP
CSB-MP475207SWP
CSB-EP475207SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Curved DNA-binding protein (cbpA) CSB-YP475208SWP
CSB-EP475208SWP
CSB-BP475208SWP
CSB-MP475208SWP
CSB-EP475208SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0225 protein ychJ (ychJ) CSB-YP475209SWP
CSB-EP475209SWP
CSB-BP475209SWP
CSB-MP475209SWP
CSB-EP475209SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP475210SWP
CSB-EP475210SWP
CSB-BP475210SWP
CSB-MP475210SWP
CSB-EP475210SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Trigger factor (tig) CSB-YP475211SWP
CSB-EP475211SWP
CSB-BP475211SWP
CSB-MP475211SWP
CSB-EP475211SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ferrous iron transport protein C (feoC) CSB-YP475212SWP
CSB-EP475212SWP
CSB-BP475212SWP
CSB-MP475212SWP
CSB-EP475212SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein smg (smg) CSB-YP475213SWP
CSB-EP475213SWP
CSB-BP475213SWP
CSB-MP475213SWP
CSB-EP475213SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP475214SWP
CSB-EP475214SWP
CSB-BP475214SWP
CSB-MP475214SWP
CSB-EP475214SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP475215SWP
CSB-EP475215SWP
CSB-BP475215SWP
CSB-MP475215SWP
CSB-EP475215SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP475216SWP
CSB-EP475216SWP
CSB-BP475216SWP
CSB-MP475216SWP
CSB-EP475216SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Hydroxylamine reductase (hcp), partial CSB-YP475217SWP
CSB-EP475217SWP
CSB-BP475217SWP
CSB-MP475217SWP
CSB-EP475217SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP475218SWP
CSB-EP475218SWP
CSB-BP475218SWP
CSB-MP475218SWP
CSB-EP475218SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0434 protein ycaR (ycaR) CSB-YP475219SWP
CSB-EP475219SWP
CSB-BP475219SWP
CSB-MP475219SWP
CSB-EP475219SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0745 protein YcgL (ycgL) CSB-YP475220SWP
CSB-EP475220SWP
CSB-BP475220SWP
CSB-MP475220SWP
CSB-EP475220SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Outer-membrane lipoprotein lolB (lolB) CSB-YP475221SWP
CSB-EP475221SWP
CSB-BP475221SWP
CSB-MP475221SWP
CSB-EP475221SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Uncharacterized protein YjeA (yjeA) CSB-YP475222SWP
CSB-EP475222SWP
CSB-BP475222SWP
CSB-MP475222SWP
CSB-EP475222SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum HTH-type transcriptional repressor NsrR (nsrR) CSB-YP475223SWP
CSB-EP475223SWP
CSB-BP475223SWP
CSB-MP475223SWP
CSB-EP475223SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP475224SWP
CSB-EP475224SWP
CSB-BP475224SWP
CSB-MP475224SWP
CSB-EP475224SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP475225SWP
CSB-EP475225SWP
CSB-BP475225SWP
CSB-MP475225SWP
CSB-EP475225SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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