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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosipho africanus 30S ribosomal protein S6 (rpsF) CSB-YP476406TNG
CSB-EP476406TNG
CSB-BP476406TNG
CSB-MP476406TNG
CSB-EP476406TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Isoleucine--tRNA ligase (ileS), partial CSB-YP476407TNG
CSB-EP476407TNG
CSB-BP476407TNG
CSB-MP476407TNG
CSB-EP476407TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus N utilization substance protein B homolog (nusB) CSB-YP476408TNG
CSB-EP476408TNG
CSB-BP476408TNG
CSB-MP476408TNG
CSB-EP476408TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Tryptophan synthase beta chain (trpB) CSB-YP476409TNG
CSB-EP476409TNG
CSB-BP476409TNG
CSB-MP476409TNG
CSB-EP476409TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S7 (rpsG) CSB-YP476410TNG
CSB-EP476410TNG
CSB-BP476410TNG
CSB-MP476410TNG
CSB-EP476410TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L2 (rplB) CSB-YP476411TNG
CSB-EP476411TNG
CSB-BP476411TNG
CSB-MP476411TNG
CSB-EP476411TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L29 (rpmC) CSB-YP476412TNG
CSB-EP476412TNG
CSB-BP476412TNG
CSB-MP476412TNG
CSB-EP476412TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP476413TNG
CSB-EP476413TNG
CSB-BP476413TNG
CSB-MP476413TNG
CSB-EP476413TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L15 (rplO) CSB-YP476414TNG
CSB-EP476414TNG
CSB-BP476414TNG
CSB-MP476414TNG
CSB-EP476414TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S4 (rpsD) CSB-YP476415TNG
CSB-EP476415TNG
CSB-BP476415TNG
CSB-MP476415TNG
CSB-EP476415TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP476416BQP1
CSB-EP476416BQP1
CSB-BP476416BQP1
CSB-MP476416BQP1
CSB-EP476416BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate--tRNA ligase (aspS), partial CSB-YP476417BQP
CSB-EP476417BQP
CSB-BP476417BQP
CSB-MP476417BQP
CSB-EP476417BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP476418BQP
CSB-EP476418BQP
CSB-BP476418BQP
CSB-MP476418BQP
CSB-EP476418BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L27 (rpmA) CSB-YP476419BQP
CSB-EP476419BQP
CSB-BP476419BQP
CSB-MP476419BQP
CSB-EP476419BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Trigger factor (tig) CSB-YP476420BQP
CSB-EP476420BQP
CSB-BP476420BQP
CSB-MP476420BQP
CSB-EP476420BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heme-degrading monooxygenase isdG (isdG) CSB-YP476421BQP
CSB-EP476421BQP
CSB-BP476421BQP
CSB-MP476421BQP
CSB-EP476421BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus HTH-type transcriptional regulator hpr (hpr) CSB-YP476422BQP
CSB-EP476422BQP
CSB-BP476422BQP
CSB-MP476422BQP
CSB-EP476422BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP476423BQP
CSB-EP476423BQP
CSB-BP476423BQP
CSB-MP476423BQP
CSB-EP476423BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-adenosylmethionine synthase (metK) CSB-YP476424BQP
CSB-EP476424BQP
CSB-BP476424BQP
CSB-MP476424BQP
CSB-EP476424BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0344 protein BCG9842_B4136 (BCG9842_B4136), partial CSB-YP476425BQP1
CSB-EP476425BQP1
CSB-BP476425BQP1
CSB-MP476425BQP1
CSB-EP476425BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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