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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 Elongation factor 4 (lepA), partial CSB-YP475126SWO
CSB-EP475126SWO
CSB-BP475126SWO
CSB-MP475126SWO
CSB-EP475126SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0133 protein ybaB (ybaB) CSB-YP475127SWO
CSB-EP475127SWO
CSB-BP475127SWO
CSB-MP475127SWO
CSB-EP475127SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 tRNA 2-selenouridine synthase (selU) CSB-YP475128SWO
CSB-EP475128SWO
CSB-BP475128SWO
CSB-MP475128SWO
CSB-EP475128SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein grpE (grpE) CSB-YP475129SWO
CSB-EP475129SWO
CSB-BP475129SWO
CSB-MP475129SWO
CSB-EP475129SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Chaperone protein TorD (torD) CSB-YP475130SWO
CSB-EP475130SWO
CSB-BP475130SWO
CSB-MP475130SWO
CSB-EP475130SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Membrane protein insertase YidC (yidC), partial CSB-YP475131SWO1
CSB-EP475131SWO1
CSB-BP475131SWO1
CSB-MP475131SWO1
CSB-EP475131SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Bifunctional protein FolD (folD) CSB-YP475132SWO
CSB-EP475132SWO
CSB-BP475132SWO
CSB-MP475132SWO
CSB-EP475132SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Carbon storage regulator (csrA) CSB-YP475133SWO
CSB-EP475133SWO
CSB-BP475133SWO
CSB-MP475133SWO
CSB-EP475133SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein CrcB homolog (crcB), partial CSB-YP475134SWO1
CSB-EP475134SWO1
CSB-BP475134SWO1
CSB-MP475134SWO1
CSB-EP475134SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) CSB-YP475135SWO
CSB-EP475135SWO
CSB-BP475135SWO
CSB-MP475135SWO
CSB-EP475135SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0061 protein ydiU (ydiU) CSB-YP475136SWO
CSB-EP475136SWO
CSB-BP475136SWO
CSB-MP475136SWO
CSB-EP475136SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 5'/3'-nucleotidase SurE (surE) CSB-YP475137SWO
CSB-EP475137SWO
CSB-BP475137SWO
CSB-MP475137SWO
CSB-EP475137SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Probable rRNA maturation factor YbeY (ybeY) CSB-YP475138SWO
CSB-EP475138SWO
CSB-BP475138SWO
CSB-MP475138SWO
CSB-EP475138SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Endonuclease 8 (nei) CSB-YP475139SWO
CSB-EP475139SWO
CSB-BP475139SWO
CSB-MP475139SWO
CSB-EP475139SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 NH (3)-dependent NAD (+) synthetase CSB-YP475140SWO
CSB-EP475140SWO
CSB-BP475140SWO
CSB-MP475140SWO
CSB-EP475140SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0756 membrane protein YeaL (yeaL), partial CSB-YP475141SWO1
CSB-EP475141SWO1
CSB-BP475141SWO1
CSB-MP475141SWO1
CSB-EP475141SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 L-fucose mutarotase (fucU) CSB-YP475142SWO
CSB-EP475142SWO
CSB-BP475142SWO
CSB-MP475142SWO
CSB-EP475142SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP475143SWO
CSB-EP475143SWO
CSB-BP475143SWO
CSB-MP475143SWO
CSB-EP475143SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 L-rhamnose mutarotase (rhaM) CSB-YP475144SWO
CSB-EP475144SWO
CSB-BP475144SWO
CSB-MP475144SWO
CSB-EP475144SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP475145SWO
CSB-EP475145SWO
CSB-BP475145SWO
CSB-MP475145SWO
CSB-EP475145SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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