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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum Cation-efflux pump FieF (fieF), partial CSB-YP475266SWP1
CSB-EP475266SWP1
CSB-BP475266SWP1
CSB-MP475266SWP1
CSB-EP475266SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum NADH pyrophosphatase (nudC) CSB-YP475267SWP
CSB-EP475267SWP
CSB-BP475267SWP
CSB-MP475267SWP
CSB-EP475267SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP475268SWP
CSB-EP475268SWP
CSB-BP475268SWP
CSB-MP475268SWP
CSB-EP475268SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP475269SWP
CSB-EP475269SWP
CSB-BP475269SWP
CSB-MP475269SWP
CSB-EP475269SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum ATPase ravA (ravA) CSB-YP475270SWP
CSB-EP475270SWP
CSB-BP475270SWP
CSB-MP475270SWP
CSB-EP475270SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Membrane protein insertase YidC (yidC), partial CSB-YP475271SWP1
CSB-EP475271SWP1
CSB-BP475271SWP1
CSB-MP475271SWP1
CSB-EP475271SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum DNA ligase B (ligB), partial CSB-YP475272SWP
CSB-EP475272SWP
CSB-BP475272SWP
CSB-MP475272SWP
CSB-EP475272SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Orotate phosphoribosyltransferase (pyrE) CSB-YP475273SWP
CSB-EP475273SWP
CSB-BP475273SWP
CSB-MP475273SWP
CSB-EP475273SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP475274SWP
CSB-EP475274SWP
CSB-BP475274SWP
CSB-MP475274SWP
CSB-EP475274SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum L-arabinose isomerase (araA) CSB-YP475275SWP
CSB-EP475275SWP
CSB-BP475275SWP
CSB-MP475275SWP
CSB-EP475275SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L28 (rpmB) CSB-YP475276SWP
CSB-EP475276SWP
CSB-BP475276SWP
CSB-MP475276SWP
CSB-EP475276SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP475277SWP
CSB-EP475277SWP
CSB-BP475277SWP
CSB-MP475277SWP
CSB-EP475277SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP475278SWP
CSB-EP475278SWP
CSB-BP475278SWP
CSB-MP475278SWP
CSB-EP475278SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Cytoplasmic trehalase (treF), partial CSB-YP475279SWP
CSB-EP475279SWP
CSB-BP475279SWP
CSB-MP475279SWP
CSB-EP475279SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Sulfurtransferase TusD (tusD) CSB-YP475280SWP
CSB-EP475280SWP
CSB-BP475280SWP
CSB-MP475280SWP
CSB-EP475280SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L22 (rplV) CSB-YP475281SWP
CSB-EP475281SWP
CSB-BP475281SWP
CSB-MP475281SWP
CSB-EP475281SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L14 (rplN) CSB-YP475282SWP
CSB-EP475282SWP
CSB-BP475282SWP
CSB-MP475282SWP
CSB-EP475282SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L18 (rplR) CSB-YP475283SWP
CSB-EP475283SWP
CSB-BP475283SWP
CSB-MP475283SWP
CSB-EP475283SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP475284SWP
CSB-EP475284SWP
CSB-BP475284SWP
CSB-MP475284SWP
CSB-EP475284SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Pantothenate synthetase (panC) CSB-YP475285SWP
CSB-EP475285SWP
CSB-BP475285SWP
CSB-MP475285SWP
CSB-EP475285SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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