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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP476226BQO
CSB-EP476226BQO
CSB-BP476226BQO
CSB-MP476226BQO
CSB-EP476226BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Arginine deiminase (arcA) CSB-YP476227BQO
CSB-EP476227BQO
CSB-BP476227BQO
CSB-MP476227BQO
CSB-EP476227BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP476228BQO
CSB-EP476228BQO
CSB-BP476228BQO
CSB-MP476228BQO
CSB-EP476228BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP476229BQO
CSB-EP476229BQO
CSB-BP476229BQO
CSB-MP476229BQO
CSB-EP476229BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylthioribose kinase (mtnK) CSB-YP476230BQO
CSB-EP476230BQO
CSB-BP476230BQO
CSB-MP476230BQO
CSB-EP476230BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Segregation and condensation protein B (scpB) CSB-YP476231BQO
CSB-EP476231BQO
CSB-BP476231BQO
CSB-MP476231BQO
CSB-EP476231BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-adenosylmethionine synthase (metK) CSB-YP476232BQO
CSB-EP476232BQO
CSB-BP476232BQO
CSB-MP476232BQO
CSB-EP476232BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP476233BQO
CSB-EP476233BQO
CSB-BP476233BQO
CSB-MP476233BQO
CSB-EP476233BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0229 protein BCB4264_A0587 (BCB4264_A0587) CSB-YP476234BQO
CSB-EP476234BQO
CSB-BP476234BQO
CSB-MP476234BQO
CSB-EP476234BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP476235BQO
CSB-EP476235BQO
CSB-BP476235BQO
CSB-MP476235BQO
CSB-EP476235BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease Z (rnz) CSB-YP476236BQO
CSB-EP476236BQO
CSB-BP476236BQO
CSB-MP476236BQO
CSB-EP476236BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional protein FolD (folD) CSB-YP476237BQO
CSB-EP476237BQO
CSB-BP476237BQO
CSB-MP476237BQO
CSB-EP476237BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP476238BQO
CSB-EP476238BQO
CSB-BP476238BQO
CSB-MP476238BQO
CSB-EP476238BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP476239BQO
CSB-EP476239BQO
CSB-BP476239BQO
CSB-MP476239BQO
CSB-EP476239BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-ribose pyranase (rbsD) CSB-YP476240BQO
CSB-EP476240BQO
CSB-BP476240BQO
CSB-MP476240BQO
CSB-EP476240BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0154 protein BCB4264_A3788 (BCB4264_A3788), partial CSB-YP476241BQO
CSB-EP476241BQO
CSB-BP476241BQO
CSB-MP476241BQO
CSB-EP476241BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA repair protein recO (recO) CSB-YP476242BQO
CSB-EP476242BQO
CSB-BP476242BQO
CSB-MP476242BQO
CSB-EP476242BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaK (dnaK), partial CSB-YP476243BQO
CSB-EP476243BQO
CSB-BP476243BQO
CSB-MP476243BQO
CSB-EP476243BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP476244BQO
CSB-EP476244BQO
CSB-BP476244BQO
CSB-MP476244BQO
CSB-EP476244BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase B chain (kdpB), partial CSB-YP476245BQO1
CSB-EP476245BQO1
CSB-BP476245BQO1
CSB-MP476245BQO1
CSB-EP476245BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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