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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Probable malate:quinone oxidoreductase (mqo) CSB-YP476426BQP
CSB-EP476426BQP
CSB-BP476426BQP
CSB-MP476426BQP
CSB-EP476426BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP476427BQP
CSB-EP476427BQP
CSB-BP476427BQP
CSB-MP476427BQP
CSB-EP476427BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP476428BQP
CSB-EP476428BQP
CSB-BP476428BQP
CSB-MP476428BQP
CSB-EP476428BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0753 protein BCG9842_B2073 (BCG9842_B2073), partial CSB-YP476429BQP
CSB-EP476429BQP
CSB-BP476429BQP
CSB-MP476429BQP
CSB-EP476429BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP476430BQP
CSB-EP476430BQP
CSB-BP476430BQP
CSB-MP476430BQP
CSB-EP476430BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP476431BQP
CSB-EP476431BQP
CSB-BP476431BQP
CSB-MP476431BQP
CSB-EP476431BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP476432BQP
CSB-EP476432BQP
CSB-BP476432BQP
CSB-MP476432BQP
CSB-EP476432BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine--tRNA ligase (metG), partial CSB-YP476433BQP
CSB-EP476433BQP
CSB-BP476433BQP
CSB-MP476433BQP
CSB-EP476433BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP476434BQP
CSB-EP476434BQP
CSB-BP476434BQP
CSB-MP476434BQP
CSB-EP476434BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Demethylmenaquinone methyltransferase (ubiE) CSB-YP476435BQP
CSB-EP476435BQP
CSB-BP476435BQP
CSB-MP476435BQP
CSB-EP476435BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pantothenate synthetase (panC) CSB-YP476436BQP
CSB-EP476436BQP
CSB-BP476436BQP
CSB-MP476436BQP
CSB-EP476436BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative sporulation transcription regulator WhiA (whiA) CSB-YP476437BQP
CSB-EP476437BQP
CSB-BP476437BQP
CSB-MP476437BQP
CSB-EP476437BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein translocase subunit SecA (secA), partial CSB-YP476438BQP
CSB-EP476438BQP
CSB-BP476438BQP
CSB-MP476438BQP
CSB-EP476438BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase epsilon chain (atpC) CSB-YP476439BQP
CSB-EP476439BQP
CSB-BP476439BQP
CSB-MP476439BQP
CSB-EP476439BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L31 type B (rpmE2) CSB-YP476440BQP
CSB-EP476440BQP
CSB-BP476440BQP
CSB-MP476440BQP
CSB-EP476440BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate--ammonia ligase (asnA) CSB-YP476441BQP
CSB-EP476441BQP
CSB-BP476441BQP
CSB-MP476441BQP
CSB-EP476441BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgB (lrgB), partial CSB-YP476442BQP1
CSB-EP476442BQP1
CSB-BP476442BQP1
CSB-MP476442BQP1
CSB-EP476442BQP1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP476443BQP
CSB-EP476443BQP
CSB-BP476443BQP
CSB-MP476443BQP
CSB-EP476443BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP476444BQP
CSB-EP476444BQP
CSB-BP476444BQP
CSB-MP476444BQP
CSB-EP476444BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein hfq (hfq) CSB-YP476445BQP
CSB-EP476445BQP
CSB-BP476445BQP
CSB-MP476445BQP
CSB-EP476445BQP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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