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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Hydrogenobaculum sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP477026HXT
CSB-EP477026HXT
CSB-BP477026HXT
CSB-MP477026HXT
CSB-EP477026HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP477027HXT
CSB-EP477027HXT
CSB-BP477027HXT
CSB-MP477027HXT
CSB-EP477027HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. Bifunctional protein FolD (folD) CSB-YP477028HXT
CSB-EP477028HXT
CSB-BP477028HXT
CSB-MP477028HXT
CSB-EP477028HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. Peptidyl-tRNA hydrolase (pth) CSB-YP477029HXT
CSB-EP477029HXT
CSB-BP477029HXT
CSB-MP477029HXT
CSB-EP477029HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. UPF0278 protein HY04AAS1_0994 (HY04AAS1_0994) CSB-YP477030HXT
CSB-EP477030HXT
CSB-BP477030HXT
CSB-MP477030HXT
CSB-EP477030HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. Histidinol-phosphate aminotransferase (hisC) CSB-YP477031HXT
CSB-EP477031HXT
CSB-BP477031HXT
CSB-MP477031HXT
CSB-EP477031HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S19 (rpsS) CSB-YP477032BYT
CSB-EP477032BYT
CSB-BP477032BYT
CSB-MP477032BYT
CSB-EP477032BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Adenylate kinase (adk) CSB-YP477033BYT
CSB-EP477033BYT
CSB-BP477033BYT
CSB-MP477033BYT
CSB-EP477033BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S16 (rpsP) CSB-YP477034BYT
CSB-EP477034BYT
CSB-BP477034BYT
CSB-MP477034BYT
CSB-EP477034BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S9 (rpsI) CSB-YP477035BYT
CSB-EP477035BYT
CSB-BP477035BYT
CSB-MP477035BYT
CSB-EP477035BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. DNA mismatch repair protein MutS (mutS), partial CSB-YP477036BYT
CSB-EP477036BYT
CSB-BP477036BYT
CSB-MP477036BYT
CSB-EP477036BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP477037BYT
CSB-EP477037BYT
CSB-BP477037BYT
CSB-MP477037BYT
CSB-EP477037BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP477038BYT
CSB-EP477038BYT
CSB-BP477038BYT
CSB-MP477038BYT
CSB-EP477038BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L33 (rpmG) CSB-YP477039BYT
CSB-EP477039BYT
CSB-BP477039BYT
CSB-MP477039BYT
CSB-EP477039BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Arginine--tRNA ligase (argS) CSB-YP477040BYT
CSB-EP477040BYT
CSB-BP477040BYT
CSB-MP477040BYT
CSB-EP477040BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP477041BYT
CSB-EP477041BYT
CSB-BP477041BYT
CSB-MP477041BYT
CSB-EP477041BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Cell division protein FtsQ (ftsQ), partial CSB-YP477042BYT
CSB-EP477042BYT
CSB-BP477042BYT
CSB-MP477042BYT
CSB-EP477042BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP477043BYT
CSB-EP477043BYT
CSB-BP477043BYT
CSB-MP477043BYT
CSB-EP477043BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Flagellar P-ring protein (flgI) CSB-YP477044BYT
CSB-EP477044BYT
CSB-BP477044BYT
CSB-MP477044BYT
CSB-EP477044BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Proline--tRNA ligase (proS) CSB-YP477045BYT
CSB-EP477045BYT
CSB-BP477045BYT
CSB-MP477045BYT
CSB-EP477045BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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