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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus ATP phosphoribosyltransferase (hisG) CSB-YP476286BQL
CSB-EP476286BQL
CSB-BP476286BQL
CSB-MP476286BQL
CSB-EP476286BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP476287BQL
CSB-EP476287BQL
CSB-BP476287BQL
CSB-MP476287BQL
CSB-EP476287BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP476288BQL
CSB-EP476288BQL
CSB-BP476288BQL
CSB-MP476288BQL
CSB-EP476288BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0302 protein BCAH187_A1683 (BCAH187_A1683) CSB-YP476289BQL
CSB-EP476289BQL
CSB-BP476289BQL
CSB-MP476289BQL
CSB-EP476289BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pantothenate synthetase (panC) CSB-YP476290BQL
CSB-EP476290BQL
CSB-BP476290BQL
CSB-MP476290BQL
CSB-EP476290BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA mismatch repair protein MutS (mutS), partial CSB-YP476291BQL
CSB-EP476291BQL
CSB-BP476291BQL
CSB-MP476291BQL
CSB-EP476291BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S2 (rpsB) CSB-YP476292BQL
CSB-EP476292BQL
CSB-BP476292BQL
CSB-MP476292BQL
CSB-EP476292BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease HII (rnhB) CSB-YP476293BQL
CSB-EP476293BQL
CSB-BP476293BQL
CSB-MP476293BQL
CSB-EP476293BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0122 protein BCAH187_A3894 (BCAH187_A3894) CSB-YP476294BQL
CSB-EP476294BQL
CSB-BP476294BQL
CSB-MP476294BQL
CSB-EP476294BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP476295BQL
CSB-EP476295BQL
CSB-BP476295BQL
CSB-MP476295BQL
CSB-EP476295BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP476296BQL
CSB-EP476296BQL
CSB-BP476296BQL
CSB-MP476296BQL
CSB-EP476296BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell division protein SepF (sepF) CSB-YP476297BQL
CSB-EP476297BQL
CSB-BP476297BQL
CSB-MP476297BQL
CSB-EP476297BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L32 (rpmF) CSB-YP476298BQL
CSB-EP476298BQL
CSB-BP476298BQL
CSB-MP476298BQL
CSB-EP476298BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0637 protein BCAH187_A4079 (BCAH187_A4079) CSB-YP476299BQL
CSB-EP476299BQL
CSB-BP476299BQL
CSB-MP476299BQL
CSB-EP476299BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP476300BQL
CSB-EP476300BQL
CSB-BP476300BQL
CSB-MP476300BQL
CSB-EP476300BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP476301BQL
CSB-EP476301BQL
CSB-BP476301BQL
CSB-MP476301BQL
CSB-EP476301BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Segregation and condensation protein B (scpB) CSB-YP476302BQL
CSB-EP476302BQL
CSB-BP476302BQL
CSB-MP476302BQL
CSB-EP476302BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NH (3)-dependent NAD (+) synthetase CSB-YP476303BQL
CSB-EP476303BQL
CSB-BP476303BQL
CSB-MP476303BQL
CSB-EP476303BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP476304BQL
CSB-EP476304BQL
CSB-BP476304BQL
CSB-MP476304BQL
CSB-EP476304BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP476305BQL
CSB-EP476305BQL
CSB-BP476305BQL
CSB-MP476305BQL
CSB-EP476305BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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