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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rabbit Cytochrome P450 1A1 (CYP1A1), partial CSB-YP006395RB
CSB-EP006395RB
CSB-BP006395RB
CSB-MP006395RB
CSB-EP006395RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Beta-nerve growth factor (NGF) CSB-YP015779CH
CSB-EP015779CH
CSB-BP015779CH
CSB-MP015779CH
CSB-EP015779CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Aspartate aminotransferase, mitochondrial (Got2) CSB-YP009681MO
CSB-EP009681MO
CSB-BP009681MO
CSB-MP009681MO
CSB-EP009681MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Fibroblast growth factor 1 (FGF1) CSB-YP008615HU
CSB-BP008615HU
CSB-MP008615HU
CSB-EP008615HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Lysosome-associated membrane glycoprotein 1 (LAMP1), partial CSB-YP012738CH1
CSB-EP012738CH1
CSB-BP012738CH1
CSB-MP012738CH1
CSB-EP012738CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Substance-K receptor (TACR2), partial CSB-YP023069BO1
CSB-EP023069BO1
CSB-BP023069BO1
CSB-MP023069BO1
CSB-EP023069BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serum amyloid A-1 protein (Saa1) CSB-YP020656MO
CSB-BP020656MO
CSB-MP020656MO
CSB-EP020656MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Farnesyl pyrophosphate synthase (Fdps) CSB-YP008563RA
CSB-EP008563RA
CSB-BP008563RA
CSB-MP008563RA
CSB-EP008563RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Large ribosomal subunit protein P1 (RPLP1) CSB-YP020340HU
CSB-BP020340HU
CSB-MP020340HU
CSB-EP020340HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA-directed RNA polymerase III subunit RPC4 (POLR3D) CSB-YP018346HU
CSB-EP018346HU
CSB-BP018346HU
CSB-MP018346HU
CSB-EP018346HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 60S ribosomal protein L7 (Rpl7) CSB-YP020302RA
CSB-EP020302RA
CSB-BP020302RA
CSB-MP020302RA
CSB-EP020302RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tetranectin (CLEC3B) CSB-YP005531HU
CSB-BP005531HU
CSB-MP005531HU
CSB-EP005531HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lupus La protein (SSB) CSB-YP022705HU
CSB-MP022705HU
CSB-EP022705HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mast/stem cell growth factor receptor Kit (Kit), partial CSB-YP012375MO
CSB-EP012375MO
CSB-BP012375MO
CSB-EP012375MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Collagen alpha-1 (II) chain (Col2a1), partial CSB-YP005739RA
CSB-EP005739RA
CSB-BP005739RA
CSB-MP005739RA
CSB-EP005739RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat T-cell surface glycoprotein CD8 beta chain (Cd8b), partial CSB-YP004967RA1
CSB-EP004967RA1
CSB-BP004967RA1
CSB-MP004967RA1
CSB-EP004967RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription factor AP-2-alpha (TFAP2A) CSB-YP023414HU
CSB-EP023414HU
CSB-BP023414HU
CSB-MP023414HU
CSB-EP023414HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat CCAAT/enhancer-binding protein alpha (Cebpa) CSB-YP005180RA
CSB-EP005180RA
CSB-BP005180RA
CSB-MP005180RA
CSB-EP005180RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Integrin beta-1 (ITGB1), partial CSB-YP011880HU1
CSB-EP011880HU1
CSB-BP011880HU1
CSB-MP011880HU1
CSB-EP011880HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Platelet-derived growth factor receptor beta (Pdgfrb), partial CSB-YP017713MO
CSB-EP017713MO
CSB-BP017713MO
CSB-MP017713MO
CSB-EP017713MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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