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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M49 Xaa-Pro dipeptidyl-peptidase (pepX), partial CSB-YP477666FNH
CSB-EP477666FNH
CSB-BP477666FNH
CSB-MP477666FNH
CSB-EP477666FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 10 kDa chaperonin (groS) CSB-YP477667FNH
CSB-EP477667FNH
CSB-BP477667FNH
CSB-MP477667FNH
CSB-EP477667FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP477668FNH
CSB-EP477668FNH
CSB-BP477668FNH
CSB-MP477668FNH
CSB-EP477668FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP477669FNH
CSB-EP477669FNH
CSB-BP477669FNH
CSB-MP477669FNH
CSB-EP477669FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L22 (rplV) CSB-YP477670FNH
CSB-EP477670FNH
CSB-BP477670FNH
CSB-MP477670FNH
CSB-EP477670FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L14 (rplN) CSB-YP477671FNH
CSB-EP477671FNH
CSB-BP477671FNH
CSB-MP477671FNH
CSB-EP477671FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 30S ribosomal protein S8 (rpsH) CSB-YP477672FNH
CSB-EP477672FNH
CSB-BP477672FNH
CSB-MP477672FNH
CSB-EP477672FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Histidine--tRNA ligase (hisS) CSB-YP477673FNH
CSB-EP477673FNH
CSB-BP477673FNH
CSB-MP477673FNH
CSB-EP477673FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 33 kDa chaperonin (hslO) CSB-YP477674FNH
CSB-EP477674FNH
CSB-BP477674FNH
CSB-MP477674FNH
CSB-EP477674FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Transcriptional repressor NrdR (nrdR) CSB-YP477675FNH
CSB-EP477675FNH
CSB-BP477675FNH
CSB-MP477675FNH
CSB-EP477675FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Segregation and condensation protein B (scpB) CSB-YP477676FNH
CSB-EP477676FNH
CSB-BP477676FNH
CSB-MP477676FNH
CSB-EP477676FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Initiation-control protein yabA (Spy49_0329) CSB-YP477677FNH
CSB-EP477677FNH
CSB-BP477677FNH
CSB-MP477677FNH
CSB-EP477677FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0346 protein Spy49_0395 (Spy49_0395) CSB-YP477678FNH
CSB-EP477678FNH
CSB-BP477678FNH
CSB-MP477678FNH
CSB-EP477678FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L19 (rplS) CSB-YP477679FNH
CSB-EP477679FNH
CSB-BP477679FNH
CSB-MP477679FNH
CSB-EP477679FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP477680FNH
CSB-EP477680FNH
CSB-BP477680FNH
CSB-MP477680FNH
CSB-EP477680FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L21 (rplU) CSB-YP477681FNH
CSB-EP477681FNH
CSB-BP477681FNH
CSB-MP477681FNH
CSB-EP477681FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP477682FNH
CSB-EP477682FNH
CSB-BP477682FNH
CSB-MP477682FNH
CSB-EP477682FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribonuclease Z (rnz) CSB-YP477683FNH
CSB-EP477683FNH
CSB-BP477683FNH
CSB-MP477683FNH
CSB-EP477683FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP477684FNH
CSB-EP477684FNH
CSB-BP477684FNH
CSB-MP477684FNH
CSB-EP477684FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0223 protein Spy49_0990c (Spy49_0990c) CSB-YP477685FNH
CSB-EP477685FNH
CSB-BP477685FNH
CSB-MP477685FNH
CSB-EP477685FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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