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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae o-succinylbenzoate synthase (menC) CSB-YP477706KBH
CSB-EP477706KBH
CSB-BP477706KBH
CSB-MP477706KBH
CSB-EP477706KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0299 membrane protein KPK_1586 (KPK_1586), partial CSB-YP477707KBH1
CSB-EP477707KBH1
CSB-BP477707KBH1
CSB-MP477707KBH1
CSB-EP477707KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP477708KBH
CSB-EP477708KBH
CSB-BP477708KBH
CSB-MP477708KBH
CSB-EP477708KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Cobalamin synthase (cobS), partial CSB-YP477709KBH1
CSB-EP477709KBH1
CSB-BP477709KBH1
CSB-MP477709KBH1
CSB-EP477709KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Aspartate--tRNA ligase (aspS), partial CSB-YP477710KBH
CSB-EP477710KBH
CSB-BP477710KBH
CSB-MP477710KBH
CSB-EP477710KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protease HtpX (htpX), partial CSB-YP477711KBH1
CSB-EP477711KBH1
CSB-BP477711KBH1
CSB-MP477711KBH1
CSB-EP477711KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0260 protein KPK_1978 (KPK_1978) CSB-YP477712KBH
CSB-EP477712KBH
CSB-BP477712KBH
CSB-MP477712KBH
CSB-EP477712KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0225 protein KPK_2103 (KPK_2103) CSB-YP477713KBH
CSB-EP477713KBH
CSB-BP477713KBH
CSB-MP477713KBH
CSB-EP477713KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Integration host factor subunit alpha (ihfA) CSB-YP477714KBH
CSB-EP477714KBH
CSB-BP477714KBH
CSB-MP477714KBH
CSB-EP477714KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (mhpB) CSB-YP477715KBH
CSB-EP477715KBH
CSB-BP477715KBH
CSB-MP477715KBH
CSB-EP477715KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Altronate oxidoreductase (uxaB) CSB-YP477716KBH
CSB-EP477716KBH
CSB-BP477716KBH
CSB-MP477716KBH
CSB-EP477716KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP477717KBH
CSB-EP477717KBH
CSB-BP477717KBH
CSB-MP477717KBH
CSB-EP477717KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP477718KBH
CSB-EP477718KBH
CSB-BP477718KBH
CSB-MP477718KBH
CSB-EP477718KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Arginine repressor (argR) CSB-YP477719KBH
CSB-EP477719KBH
CSB-BP477719KBH
CSB-MP477719KBH
CSB-EP477719KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0042 nucleotide-binding protein KPK_0506 (KPK_0506) CSB-YP477720KBH
CSB-EP477720KBH
CSB-BP477720KBH
CSB-MP477720KBH
CSB-EP477720KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphoglucosamine mutase (glmM) CSB-YP477721KBH
CSB-EP477721KBH
CSB-BP477721KBH
CSB-MP477721KBH
CSB-EP477721KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0102 protein KPK_0567 (KPK_0567) CSB-YP477722KBH
CSB-EP477722KBH
CSB-BP477722KBH
CSB-MP477722KBH
CSB-EP477722KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0259 membrane protein KPK_3195 (KPK_3195), partial CSB-YP477723KBH1
CSB-EP477723KBH1
CSB-BP477723KBH1
CSB-MP477723KBH1
CSB-EP477723KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glycerol kinase (glpK), partial CSB-YP477724KBH
CSB-EP477724KBH
CSB-BP477724KBH
CSB-MP477724KBH
CSB-EP477724KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L33 (rpmG) CSB-YP477725KBH
CSB-EP477725KBH
CSB-BP477725KBH
CSB-MP477725KBH
CSB-EP477725KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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