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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae Protein-export protein secB (secB) CSB-YP477726KBH
CSB-EP477726KBH
CSB-BP477726KBH
CSB-MP477726KBH
CSB-EP477726KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP477727KBH
CSB-EP477727KBH
CSB-BP477727KBH
CSB-MP477727KBH
CSB-EP477727KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Sulfurtransferase TusA (tusA) CSB-YP477728KBH
CSB-EP477728KBH
CSB-BP477728KBH
CSB-MP477728KBH
CSB-EP477728KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Rhomboid protease glpG (glpG), partial CSB-YP477729KBH1
CSB-EP477729KBH1
CSB-BP477729KBH1
CSB-MP477729KBH1
CSB-EP477729KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Uronate isomerase (uxaC) CSB-YP477730KBH
CSB-EP477730KBH
CSB-BP477730KBH
CSB-MP477730KBH
CSB-EP477730KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 30S ribosomal protein S21 (rpsU) CSB-YP477731KBH
CSB-EP477731KBH
CSB-BP477731KBH
CSB-MP477731KBH
CSB-EP477731KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Urease accessory protein UreE (ureE) CSB-YP477732KBH
CSB-EP477732KBH
CSB-BP477732KBH
CSB-MP477732KBH
CSB-EP477732KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Undecaprenyl-diphosphatase (uppP), partial CSB-YP477733KBH
CSB-EP477733KBH
CSB-BP477733KBH
CSB-MP477733KBH
CSB-EP477733KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0441 protein KPK_0672 (KPK_0672) CSB-YP477734KBH
CSB-EP477734KBH
CSB-BP477734KBH
CSB-MP477734KBH
CSB-EP477734KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0301 protein KPK_0728 (KPK_0728) CSB-YP477735KBH
CSB-EP477735KBH
CSB-BP477735KBH
CSB-MP477735KBH
CSB-EP477735KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA-modifying protein ygfZ (KPK_0772) CSB-YP477736KBH
CSB-EP477736KBH
CSB-BP477736KBH
CSB-MP477736KBH
CSB-EP477736KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable propionate kinase (pduW) CSB-YP477737KBH
CSB-EP477737KBH
CSB-BP477737KBH
CSB-MP477737KBH
CSB-EP477737KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae L-fucose mutarotase (fucU) CSB-YP477738KBH
CSB-EP477738KBH
CSB-BP477738KBH
CSB-MP477738KBH
CSB-EP477738KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Adenylyl-sulfate kinase (cysC) CSB-YP477739KBH
CSB-EP477739KBH
CSB-BP477739KBH
CSB-MP477739KBH
CSB-EP477739KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 5'/3'-nucleotidase SurE (surE) CSB-YP477740KBH
CSB-EP477740KBH
CSB-BP477740KBH
CSB-MP477740KBH
CSB-EP477740KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP477741KBH
CSB-EP477741KBH
CSB-BP477741KBH
CSB-MP477741KBH
CSB-EP477741KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP477742KBH
CSB-EP477742KBH
CSB-BP477742KBH
CSB-MP477742KBH
CSB-EP477742KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP477743KBH
CSB-EP477743KBH
CSB-BP477743KBH
CSB-MP477743KBH
CSB-EP477743KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glutamate--tRNA ligase (gltX) CSB-YP477744KBH
CSB-EP477744KBH
CSB-BP477744KBH
CSB-MP477744KBH
CSB-EP477744KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Peptidyl-tRNA hydrolase (pth) CSB-YP477745KBH
CSB-EP477745KBH
CSB-BP477745KBH
CSB-MP477745KBH
CSB-EP477745KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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