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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP477786KBH
CSB-EP477786KBH
CSB-BP477786KBH
CSB-MP477786KBH
CSB-EP477786KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Elongation factor P (efp) CSB-YP477787KBH
CSB-EP477787KBH
CSB-BP477787KBH
CSB-MP477787KBH
CSB-EP477787KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus UPF0758 protein COPRO5265_1522 (COPRO5265_1522) CSB-YP477788DWR
CSB-EP477788DWR
CSB-BP477788DWR
CSB-MP477788DWR
CSB-EP477788DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus DNA ligase (ligA), partial CSB-YP477789DWR
CSB-EP477789DWR
CSB-BP477789DWR
CSB-MP477789DWR
CSB-EP477789DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus SsrA-binding protein (smpB) CSB-YP477790DWR
CSB-EP477790DWR
CSB-BP477790DWR
CSB-MP477790DWR
CSB-EP477790DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 10 kDa chaperonin (groS) CSB-YP477791DWR
CSB-EP477791DWR
CSB-BP477791DWR
CSB-MP477791DWR
CSB-EP477791DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP477792DWR
CSB-EP477792DWR
CSB-BP477792DWR
CSB-MP477792DWR
CSB-EP477792DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Peptidyl-tRNA hydrolase (pth) CSB-YP477793DWR
CSB-EP477793DWR
CSB-BP477793DWR
CSB-MP477793DWR
CSB-EP477793DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 50S ribosomal protein L7/L12 (rplL) CSB-YP477794DWR
CSB-EP477794DWR
CSB-BP477794DWR
CSB-MP477794DWR
CSB-EP477794DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 50S ribosomal protein L17 (rplQ) CSB-YP477795DWR
CSB-EP477795DWR
CSB-BP477795DWR
CSB-MP477795DWR
CSB-EP477795DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 30S ribosomal protein S8 (rpsH) CSB-YP477796DWR
CSB-EP477796DWR
CSB-BP477796DWR
CSB-MP477796DWR
CSB-EP477796DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Probable transaldolase (tal) CSB-YP477797DWR
CSB-EP477797DWR
CSB-BP477797DWR
CSB-MP477797DWR
CSB-EP477797DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum CinA-like protein (DICTH_1766) CSB-YP477798DKI
CSB-EP477798DKI
CSB-BP477798DKI
CSB-MP477798DKI
CSB-EP477798DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum ATP synthase gamma chain (atpG) CSB-YP477799DKI
CSB-EP477799DKI
CSB-BP477799DKI
CSB-MP477799DKI
CSB-EP477799DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Tryptophan synthase alpha chain (trpA) CSB-YP477800DKI
CSB-EP477800DKI
CSB-BP477800DKI
CSB-MP477800DKI
CSB-EP477800DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L31 (rpmE) CSB-YP477801DKI
CSB-EP477801DKI
CSB-BP477801DKI
CSB-MP477801DKI
CSB-EP477801DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Acetate kinase (ackA) CSB-YP477802DKI
CSB-EP477802DKI
CSB-BP477802DKI
CSB-MP477802DKI
CSB-EP477802DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP477803DKI
CSB-EP477803DKI
CSB-BP477803DKI
CSB-MP477803DKI
CSB-EP477803DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S19 (rpsS) CSB-YP477804DKI
CSB-EP477804DKI
CSB-BP477804DKI
CSB-MP477804DKI
CSB-EP477804DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L24 (rplX) CSB-YP477805DKI
CSB-EP477805DKI
CSB-BP477805DKI
CSB-MP477805DKI
CSB-EP477805DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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