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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella heidelberg Low affinity potassium transport system protein kup (kup), partial CSB-YP479179SWQ
CSB-EP479179SWQ
CSB-BP479179SWQ
CSB-MP479179SWQ
CSB-EP479179SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 4-alpha-L-fucosyltransferase (wecF) CSB-YP479180SWQ
CSB-EP479180SWQ
CSB-BP479180SWQ
CSB-MP479180SWQ
CSB-EP479180SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP479181SWQ
CSB-EP479181SWQ
CSB-BP479181SWQ
CSB-MP479181SWQ
CSB-EP479181SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP479182SWQ
CSB-EP479182SWQ
CSB-BP479182SWQ
CSB-MP479182SWQ
CSB-EP479182SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ribonuclease BN (rbn) CSB-YP479183SWQ
CSB-EP479183SWQ
CSB-BP479183SWQ
CSB-MP479183SWQ
CSB-EP479183SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP479184SWQ
CSB-EP479184SWQ
CSB-BP479184SWQ
CSB-MP479184SWQ
CSB-EP479184SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable GTP-binding protein EngB (engB) CSB-YP479185SWQ
CSB-EP479185SWQ
CSB-BP479185SWQ
CSB-MP479185SWQ
CSB-EP479185SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Rhamnulokinase (rhaB) CSB-YP479186SWQ
CSB-EP479186SWQ
CSB-BP479186SWQ
CSB-MP479186SWQ
CSB-EP479186SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0194 membrane protein ybhG (ybhG) CSB-YP479187SWQ
CSB-EP479187SWQ
CSB-BP479187SWQ
CSB-MP479187SWQ
CSB-EP479187SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0115 protein yfcN (yfcN) CSB-YP479188SWQ
CSB-EP479188SWQ
CSB-BP479188SWQ
CSB-MP479188SWQ
CSB-EP479188SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg DNA ligase (ligA), partial CSB-YP479189SWQ
CSB-EP479189SWQ
CSB-BP479189SWQ
CSB-MP479189SWQ
CSB-EP479189SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Cell division protein ZapB (zapB) CSB-YP479190SWQ
CSB-EP479190SWQ
CSB-BP479190SWQ
CSB-MP479190SWQ
CSB-EP479190SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable fructose-6-phosphate aldolase (fsa) CSB-YP479191SWQ
CSB-EP479191SWQ
CSB-BP479191SWQ
CSB-MP479191SWQ
CSB-EP479191SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Pantothenate kinase (coaA) CSB-YP479192SWQ
CSB-EP479192SWQ
CSB-BP479192SWQ
CSB-MP479192SWQ
CSB-EP479192SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Endonuclease V (nfi) CSB-YP479193SWQ
CSB-EP479193SWQ
CSB-BP479193SWQ
CSB-MP479193SWQ
CSB-EP479193SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP479194SWQ
CSB-EP479194SWQ
CSB-BP479194SWQ
CSB-MP479194SWQ
CSB-EP479194SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP479195SWQ
CSB-EP479195SWQ
CSB-BP479195SWQ
CSB-MP479195SWQ
CSB-EP479195SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0370 protein ypfN (ypfN), partial CSB-YP479196SWQ
CSB-EP479196SWQ
CSB-BP479196SWQ
CSB-MP479196SWQ
CSB-EP479196SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP479197SWQ
CSB-EP479197SWQ
CSB-BP479197SWQ
CSB-MP479197SWQ
CSB-EP479197SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg HTH-type transcriptional regulator iscR (iscR) CSB-YP479198SWQ
CSB-EP479198SWQ
CSB-BP479198SWQ
CSB-MP479198SWQ
CSB-EP479198SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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