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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anaeromyxobacter sp. S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP479379BYT
CSB-EP479379BYT
CSB-BP479379BYT
CSB-MP479379BYT
CSB-EP479379BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S20 (rpsT) CSB-YP479380BYT
CSB-EP479380BYT
CSB-BP479380BYT
CSB-MP479380BYT
CSB-EP479380BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP479381BYT
CSB-EP479381BYT
CSB-BP479381BYT
CSB-MP479381BYT
CSB-EP479381BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Heat-inducible transcription repressor HrcA (hrcA) CSB-YP479382BYT
CSB-EP479382BYT
CSB-BP479382BYT
CSB-MP479382BYT
CSB-EP479382BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP479383BYT
CSB-EP479383BYT
CSB-BP479383BYT
CSB-MP479383BYT
CSB-EP479383BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP479384BYT
CSB-EP479384BYT
CSB-BP479384BYT
CSB-MP479384BYT
CSB-EP479384BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L25 (rplY) CSB-YP479385BYT
CSB-EP479385BYT
CSB-BP479385BYT
CSB-MP479385BYT
CSB-EP479385BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Glycogen synthase (glgA) CSB-YP479386BYT
CSB-EP479386BYT
CSB-BP479386BYT
CSB-MP479386BYT
CSB-EP479386BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Gamma-glutamyl phosphate reductase (proA) CSB-YP479387BYT
CSB-EP479387BYT
CSB-BP479387BYT
CSB-MP479387BYT
CSB-EP479387BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Altered inheritance of mitochondria protein 4 (AIM4) CSB-YP479388KBK
CSB-EP479388KBK
CSB-BP479388KBK
CSB-MP479388KBK
CSB-EP479388KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin A10 CSB-YP479389MPJ
CSB-EP479389MPJ
CSB-BP479389MPJ
CSB-MP479389MPJ
CSB-EP479389MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin A15 CSB-YP479390MPJ
CSB-EP479390MPJ
CSB-BP479390MPJ
CSB-MP479390MPJ
CSB-EP479390MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin B1 CSB-YP479391MPJ
CSB-EP479391MPJ
CSB-BP479391MPJ
CSB-MP479391MPJ
CSB-EP479391MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin B7 CSB-YP479392MPJ
CSB-EP479392MPJ
CSB-BP479392MPJ
CSB-MP479392MPJ
CSB-EP479392MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative helicase V13, partial CSB-YP479393FKV
CSB-EP479393FKV
CSB-BP479393FKV
CSB-MP479393FKV
CSB-EP479393FKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein V21 (ORF21) CSB-YP479394FKV
CSB-EP479394FKV
CSB-BP479394FKV
CSB-MP479394FKV
CSB-EP479394FKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Cysteine desulfuration protein sufE (sufE) CSB-YP479395SWT
CSB-EP479395SWT
CSB-BP479395SWT
CSB-MP479395SWT
CSB-EP479395SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP479396SWT
CSB-EP479396SWT
CSB-BP479396SWT
CSB-MP479396SWT
CSB-EP479396SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0756 membrane protein YeaL (yeaL) CSB-YP479397SWT
CSB-EP479397SWT
CSB-BP479397SWT
CSB-MP479397SWT
CSB-EP479397SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Phosphopentomutase (deoB) CSB-YP479398SWT
CSB-EP479398SWT
CSB-BP479398SWT
CSB-MP479398SWT
CSB-EP479398SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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