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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella newport Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP479159SWS
CSB-EP479159SWS
CSB-BP479159SWS
CSB-MP479159SWS
CSB-EP479159SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP479160SWS
CSB-EP479160SWS
CSB-BP479160SWS
CSB-MP479160SWS
CSB-EP479160SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0509 protein yciZ (yciZ) CSB-YP479161SWS
CSB-EP479161SWS
CSB-BP479161SWS
CSB-MP479161SWS
CSB-EP479161SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0102 protein yraN (yraN) CSB-YP479162SWS
CSB-EP479162SWS
CSB-BP479162SWS
CSB-MP479162SWS
CSB-EP479162SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP479163SWS
CSB-EP479163SWS
CSB-BP479163SWS
CSB-MP479163SWS
CSB-EP479163SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP479164SWS
CSB-EP479164SWS
CSB-BP479164SWS
CSB-MP479164SWS
CSB-EP479164SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 30S ribosomal protein S9 (rpsI) CSB-YP479165SWS
CSB-EP479165SWS
CSB-BP479165SWS
CSB-MP479165SWS
CSB-EP479165SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Phosphoheptose isomerase (gmhA) CSB-YP479166SWQ
CSB-EP479166SWQ
CSB-BP479166SWQ
CSB-MP479166SWQ
CSB-EP479166SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP479167SWQ
CSB-EP479167SWQ
CSB-BP479167SWQ
CSB-MP479167SWQ
CSB-EP479167SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Glycogen synthase (glgA) CSB-YP479168SWQ
CSB-EP479168SWQ
CSB-BP479168SWQ
CSB-MP479168SWQ
CSB-EP479168SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Shikimate kinase 2 (aroL) CSB-YP479169SWQ
CSB-EP479169SWQ
CSB-BP479169SWQ
CSB-MP479169SWQ
CSB-EP479169SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP479170SWQ
CSB-EP479170SWQ
CSB-BP479170SWQ
CSB-MP479170SWQ
CSB-EP479170SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP479171SWQ
CSB-EP479171SWQ
CSB-BP479171SWQ
CSB-MP479171SWQ
CSB-EP479171SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP479172SWQ
CSB-EP479172SWQ
CSB-BP479172SWQ
CSB-MP479172SWQ
CSB-EP479172SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0758 protein yicR (yicR) CSB-YP479173SWQ
CSB-EP479173SWQ
CSB-BP479173SWQ
CSB-MP479173SWQ
CSB-EP479173SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP479174SWQ
CSB-EP479174SWQ
CSB-BP479174SWQ
CSB-MP479174SWQ
CSB-EP479174SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Allantoinase (allB) CSB-YP479175SWQ
CSB-EP479175SWQ
CSB-BP479175SWQ
CSB-MP479175SWQ
CSB-EP479175SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg D-galactonate dehydratase (dgoD) CSB-YP479176SWQ
CSB-EP479176SWQ
CSB-BP479176SWQ
CSB-MP479176SWQ
CSB-EP479176SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Nucleoid-associated protein YejK (yejK) CSB-YP479177SWQ
CSB-EP479177SWQ
CSB-BP479177SWQ
CSB-MP479177SWQ
CSB-EP479177SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Multidrug resistance protein MdtL (mdtL), partial CSB-YP479178SWQ
CSB-EP479178SWQ
CSB-BP479178SWQ
CSB-MP479178SWQ
CSB-EP479178SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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