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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella heidelberg 50S ribosomal protein L14 (rplN) CSB-YP479239SWQ
CSB-EP479239SWQ
CSB-BP479239SWQ
CSB-MP479239SWQ
CSB-EP479239SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L22 (rplV) CSB-YP479240SWQ
CSB-EP479240SWQ
CSB-BP479240SWQ
CSB-MP479240SWQ
CSB-EP479240SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 30S ribosomal protein S12 (rpsL) CSB-YP479241SWQ
CSB-EP479241SWQ
CSB-BP479241SWQ
CSB-MP479241SWQ
CSB-EP479241SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ferrous iron transport protein C (feoC) CSB-YP479242SWQ
CSB-EP479242SWQ
CSB-BP479242SWQ
CSB-MP479242SWQ
CSB-EP479242SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Transcriptional repressor NrdR (nrdR) CSB-YP479243SWV
CSB-EP479243SWV
CSB-BP479243SWV
CSB-MP479243SWV
CSB-EP479243SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0234 protein yajQ (yajQ) CSB-YP479244SWV
CSB-EP479244SWV
CSB-BP479244SWV
CSB-MP479244SWV
CSB-EP479244SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Putative transport protein YidE (yidE), partial CSB-YP479245SWV
CSB-EP479245SWV
CSB-BP479245SWV
CSB-MP479245SWV
CSB-EP479245SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Multidrug resistance protein MdtL (mdtL), partial CSB-YP479246SWV
CSB-EP479246SWV
CSB-BP479246SWV
CSB-MP479246SWV
CSB-EP479246SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP479247SWV
CSB-EP479247SWV
CSB-BP479247SWV
CSB-MP479247SWV
CSB-EP479247SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Bifunctional protein FolD (folD) CSB-YP479248SWV
CSB-EP479248SWV
CSB-BP479248SWV
CSB-MP479248SWV
CSB-EP479248SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP479249SWV
CSB-EP479249SWV
CSB-BP479249SWV
CSB-MP479249SWV
CSB-EP479249SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein CrcB homolog (crcB), partial CSB-YP479250SWV1
CSB-EP479250SWV1
CSB-BP479250SWV1
CSB-MP479250SWV1
CSB-EP479250SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0352 protein yejL (yejL) CSB-YP479251SWV
CSB-EP479251SWV
CSB-BP479251SWV
CSB-MP479251SWV
CSB-EP479251SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP479252SWV
CSB-EP479252SWV
CSB-BP479252SWV
CSB-MP479252SWV
CSB-EP479252SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP479253SWV1
CSB-EP479253SWV1
CSB-BP479253SWV1
CSB-MP479253SWV1
CSB-EP479253SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable GTP-binding protein EngB (engB) CSB-YP479254SWV
CSB-EP479254SWV
CSB-BP479254SWV
CSB-MP479254SWV
CSB-EP479254SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund HTH-type transcriptional activator rhaR (rhaR) CSB-YP479255SWV
CSB-EP479255SWV
CSB-BP479255SWV
CSB-MP479255SWV
CSB-EP479255SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Met repressor (metJ) CSB-YP479256SWV
CSB-EP479256SWV
CSB-BP479256SWV
CSB-MP479256SWV
CSB-EP479256SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glucosamine-6-phosphate deaminase (nagB) CSB-YP479257SWV
CSB-EP479257SWV
CSB-BP479257SWV
CSB-MP479257SWV
CSB-EP479257SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable fructose-6-phosphate aldolase (fsa) CSB-YP479258SWV
CSB-EP479258SWV
CSB-BP479258SWV
CSB-MP479258SWV
CSB-EP479258SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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