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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Macrovipera lebetina C-type lectin B9 CSB-YP480379MPJ
CSB-EP480379MPJ
CSB-BP480379MPJ
CSB-MP480379MPJ
CSB-EP480379MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein V17 (ORF17) CSB-YP480380FKV
CSB-EP480380FKV
CSB-BP480380FKV
CSB-MP480380FKV
CSB-EP480380FKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein quiver (qvr) CSB-YP480381DLU
CSB-EP480381DLU
CSB-BP480381DLU
CSB-MP480381DLU
CSB-EP480381DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Putative phosphotransferase ydiA (ydiA) CSB-YP480382SWT
CSB-EP480382SWT
CSB-BP480382SWT
CSB-MP480382SWT
CSB-EP480382SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP480383SWT
CSB-EP480383SWT
CSB-BP480383SWT
CSB-MP480383SWT
CSB-EP480383SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Succinylglutamate desuccinylase (astE) CSB-YP480384SWT
CSB-EP480384SWT
CSB-BP480384SWT
CSB-MP480384SWT
CSB-EP480384SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Succinylornithine transaminase (astC) CSB-YP480385SWT
CSB-EP480385SWT
CSB-BP480385SWT
CSB-MP480385SWT
CSB-EP480385SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Non-canonical purine NTP phosphatase (yjjX) CSB-YP480386SWT
CSB-EP480386SWT
CSB-BP480386SWT
CSB-MP480386SWT
CSB-EP480386SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP480387SWT
CSB-EP480387SWT
CSB-BP480387SWT
CSB-MP480387SWT
CSB-EP480387SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP480388SWT
CSB-EP480388SWT
CSB-BP480388SWT
CSB-MP480388SWT
CSB-EP480388SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Co-chaperone protein hscB (hscB) CSB-YP480389SWT
CSB-EP480389SWT
CSB-BP480389SWT
CSB-MP480389SWT
CSB-EP480389SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP480390SWT
CSB-EP480390SWT
CSB-BP480390SWT
CSB-MP480390SWT
CSB-EP480390SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP480391SWT
CSB-EP480391SWT
CSB-BP480391SWT
CSB-MP480391SWT
CSB-EP480391SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Glutamate--tRNA ligase (gltX) CSB-YP480392SWT
CSB-EP480392SWT
CSB-BP480392SWT
CSB-MP480392SWT
CSB-EP480392SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0115 protein yfcN (yfcN) CSB-YP480393SWT
CSB-EP480393SWT
CSB-BP480393SWT
CSB-MP480393SWT
CSB-EP480393SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Glucans biosynthesis protein G (mdoG) CSB-YP480394SWT
CSB-EP480394SWT
CSB-BP480394SWT
CSB-MP480394SWT
CSB-EP480394SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) CSB-YP480395SWT
CSB-EP480395SWT
CSB-BP480395SWT
CSB-MP480395SWT
CSB-EP480395SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP480396SWT
CSB-EP480396SWT
CSB-BP480396SWT
CSB-MP480396SWT
CSB-EP480396SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP480397SWT
CSB-EP480397SWT
CSB-BP480397SWT
CSB-MP480397SWT
CSB-EP480397SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Ribosomal protein S6 modification protein (rimK) CSB-YP480398SWT
CSB-EP480398SWT
CSB-BP480398SWT
CSB-MP480398SWT
CSB-EP480398SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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