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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund 30S ribosomal protein S10 (rpsJ) CSB-YP479299SWV
CSB-EP479299SWV
CSB-BP479299SWV
CSB-MP479299SWV
CSB-EP479299SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Sulfurtransferase TusD (tusD) CSB-YP479300SWV
CSB-EP479300SWV
CSB-BP479300SWV
CSB-MP479300SWV
CSB-EP479300SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein translocase subunit SecA (secA), partial CSB-YP479301SWV
CSB-EP479301SWV
CSB-BP479301SWV
CSB-MP479301SWV
CSB-EP479301SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Pantothenate synthetase (panC) CSB-YP479302SWV
CSB-EP479302SWV
CSB-BP479302SWV
CSB-MP479302SWV
CSB-EP479302SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP479303SWV
CSB-EP479303SWV
CSB-BP479303SWV
CSB-MP479303SWV
CSB-EP479303SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP479304SWV
CSB-EP479304SWV
CSB-BP479304SWV
CSB-MP479304SWV
CSB-EP479304SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-amino acid dehydrogenase small subunit (dadA) CSB-YP479305SWV
CSB-EP479305SWV
CSB-BP479305SWV
CSB-MP479305SWV
CSB-EP479305SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cell division topological specificity factor (minE) CSB-YP479306SWV
CSB-EP479306SWV
CSB-BP479306SWV
CSB-MP479306SWV
CSB-EP479306SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP479307SWV
CSB-EP479307SWV
CSB-BP479307SWV
CSB-MP479307SWV
CSB-EP479307SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Rhomboid protease glpG (glpG), partial CSB-YP479308SWV1
CSB-EP479308SWV1
CSB-BP479308SWV1
CSB-MP479308SWV1
CSB-EP479308SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP479309SWV
CSB-EP479309SWV
CSB-BP479309SWV
CSB-MP479309SWV
CSB-EP479309SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Phosphoheptose isomerase (gmhA) CSB-YP479310SWV
CSB-EP479310SWV
CSB-BP479310SWV
CSB-MP479310SWV
CSB-EP479310SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund tRNA (mo5U34)-methyltransferase CSB-YP479311SWV
CSB-EP479311SWV
CSB-BP479311SWV
CSB-MP479311SWV
CSB-EP479311SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cytoplasmic trehalase (treF), partial CSB-YP479312SWV
CSB-EP479312SWV
CSB-BP479312SWV
CSB-MP479312SWV
CSB-EP479312SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glutamate 5-kinase (proB) CSB-YP479313SWV
CSB-EP479313SWV
CSB-BP479313SWV
CSB-MP479313SWV
CSB-EP479313SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP479314SWV
CSB-EP479314SWV
CSB-BP479314SWV
CSB-MP479314SWV
CSB-EP479314SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP479315SWV
CSB-EP479315SWV
CSB-BP479315SWV
CSB-MP479315SWV
CSB-EP479315SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0758 protein yicR (yicR) CSB-YP479316SWV
CSB-EP479316SWV
CSB-BP479316SWV
CSB-MP479316SWV
CSB-EP479316SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 10 kDa chaperonin (groS) CSB-YP479317FMK
CSB-EP479317FMK
CSB-BP479317FMK
CSB-MP479317FMK
CSB-EP479317FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Protein RecA (recA) CSB-YP479318FMK
CSB-EP479318FMK
CSB-BP479318FMK
CSB-MP479318FMK
CSB-EP479318FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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