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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Hydrogenobaculum sp. Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP479359HXT1
CSB-EP479359HXT1
CSB-BP479359HXT1
CSB-MP479359HXT1
CSB-EP479359HXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L3 (rplC) CSB-YP479360BYT
CSB-EP479360BYT
CSB-BP479360BYT
CSB-MP479360BYT
CSB-EP479360BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L35 (rpmI) CSB-YP479361BYT
CSB-EP479361BYT
CSB-BP479361BYT
CSB-MP479361BYT
CSB-EP479361BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L4 (rplD) CSB-YP479362BYT
CSB-EP479362BYT
CSB-BP479362BYT
CSB-MP479362BYT
CSB-EP479362BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L14 (rplN) CSB-YP479363BYT
CSB-EP479363BYT
CSB-BP479363BYT
CSB-MP479363BYT
CSB-EP479363BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L36 (rpmJ) CSB-YP479364BYT
CSB-EP479364BYT
CSB-BP479364BYT
CSB-MP479364BYT
CSB-EP479364BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L19 (rplS) CSB-YP479365BYT
CSB-EP479365BYT
CSB-BP479365BYT
CSB-MP479365BYT
CSB-EP479365BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 8-amino-7-oxononanoate synthase (bioF) CSB-YP479366BYT
CSB-EP479366BYT
CSB-BP479366BYT
CSB-MP479366BYT
CSB-EP479366BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. LexA repressor (lexA) CSB-YP479367BYT
CSB-EP479367BYT
CSB-BP479367BYT
CSB-MP479367BYT
CSB-EP479367BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. UPF0133 protein AnaeK_3694 (AnaeK_3694) CSB-YP479368BYT
CSB-EP479368BYT
CSB-BP479368BYT
CSB-MP479368BYT
CSB-EP479368BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L1 (rplA) CSB-YP479369BYT
CSB-EP479369BYT
CSB-BP479369BYT
CSB-MP479369BYT
CSB-EP479369BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 5'-nucleotidase surE (surE) CSB-YP479370BYT
CSB-EP479370BYT
CSB-BP479370BYT
CSB-MP479370BYT
CSB-EP479370BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. D-alanine--D-alanine ligase (ddl) CSB-YP479371BYT
CSB-EP479371BYT
CSB-BP479371BYT
CSB-MP479371BYT
CSB-EP479371BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Serine--tRNA ligase (serS) CSB-YP479372BYT
CSB-EP479372BYT
CSB-BP479372BYT
CSB-MP479372BYT
CSB-EP479372BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP479373BYT
CSB-EP479373BYT
CSB-BP479373BYT
CSB-MP479373BYT
CSB-EP479373BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Tyrosine--tRNA ligase (tyrS) CSB-YP479374BYT
CSB-EP479374BYT
CSB-BP479374BYT
CSB-MP479374BYT
CSB-EP479374BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Adenylosuccinate synthetase (purA) CSB-YP479375BYT
CSB-EP479375BYT
CSB-BP479375BYT
CSB-MP479375BYT
CSB-EP479375BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Aspartate 1-decarboxylase (panD) CSB-YP479376BYT
CSB-EP479376BYT
CSB-BP479376BYT
CSB-MP479376BYT
CSB-EP479376BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP479377BYT
CSB-EP479377BYT
CSB-BP479377BYT
CSB-MP479377BYT
CSB-EP479377BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP479378BYT
CSB-EP479378BYT
CSB-BP479378BYT
CSB-MP479378BYT
CSB-EP479378BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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