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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio fischeri UPF0234 protein VFMJ11_1323 (VFMJ11_1323) CSB-YP480659VFC
CSB-EP480659VFC
CSB-BP480659VFC
CSB-MP480659VFC
CSB-EP480659VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Dihydroorotate dehydrogenase (quinone) CSB-YP480660VFC
CSB-EP480660VFC
CSB-BP480660VFC
CSB-MP480660VFC
CSB-EP480660VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0397 protein VFMJ11_1662 (VFMJ11_1662), partial CSB-YP480661VFC1
CSB-EP480661VFC1
CSB-BP480661VFC1
CSB-MP480661VFC1
CSB-EP480661VFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Methylglyoxal synthase (mgsA) CSB-YP480662VFC
CSB-EP480662VFC
CSB-BP480662VFC
CSB-MP480662VFC
CSB-EP480662VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP480663VFC
CSB-EP480663VFC
CSB-BP480663VFC
CSB-MP480663VFC
CSB-EP480663VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0758 protein VFMJ11_0123 (VFMJ11_0123) CSB-YP480664VFC
CSB-EP480664VFC
CSB-BP480664VFC
CSB-MP480664VFC
CSB-EP480664VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0133 protein VFMJ11_1810 (VFMJ11_1810) CSB-YP480665VFC
CSB-EP480665VFC
CSB-BP480665VFC
CSB-MP480665VFC
CSB-EP480665VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Cell division topological specificity factor (minE) CSB-YP480666VFC
CSB-EP480666VFC
CSB-BP480666VFC
CSB-MP480666VFC
CSB-EP480666VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Protein slyX homolog (slyX) CSB-YP480667VFC
CSB-EP480667VFC
CSB-BP480667VFC
CSB-MP480667VFC
CSB-EP480667VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L2 (rplB) CSB-YP480668VFC
CSB-EP480668VFC
CSB-BP480668VFC
CSB-MP480668VFC
CSB-EP480668VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L18 (rplR) CSB-YP480669VFC
CSB-EP480669VFC
CSB-BP480669VFC
CSB-MP480669VFC
CSB-EP480669VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Cytidylate kinase (cmk) CSB-YP480670VFC
CSB-EP480670VFC
CSB-BP480670VFC
CSB-MP480670VFC
CSB-EP480670VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri High frequency lysogenization protein HflD homolog (hflD) CSB-YP480671VFC
CSB-EP480671VFC
CSB-BP480671VFC
CSB-MP480671VFC
CSB-EP480671VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP480672VFC
CSB-EP480672VFC
CSB-BP480672VFC
CSB-MP480672VFC
CSB-EP480672VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L17 (rplQ) CSB-YP480673VFC
CSB-EP480673VFC
CSB-BP480673VFC
CSB-MP480673VFC
CSB-EP480673VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Uracil phosphoribosyltransferase (upp) CSB-YP480674VFC
CSB-EP480674VFC
CSB-BP480674VFC
CSB-MP480674VFC
CSB-EP480674VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chaperone protein DnaK (dnaK), partial CSB-YP480675SWN
CSB-EP480675SWN
CSB-BP480675SWN
CSB-MP480675SWN
CSB-EP480675SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Crotonobetainyl-CoA dehydrogenase (caiA) CSB-YP480676SWN
CSB-EP480676SWN
CSB-BP480676SWN
CSB-MP480676SWN
CSB-EP480676SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0060 membrane protein ynfA (ynfA), partial CSB-YP480677SWN1
CSB-EP480677SWN1
CSB-BP480677SWN1
CSB-MP480677SWN1
CSB-EP480677SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Putrescine aminotransferase (patA) CSB-YP480678SWN
CSB-EP480678SWN
CSB-BP480678SWN
CSB-MP480678SWN
CSB-EP480678SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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