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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella agona NH (3)-dependent NAD (+) synthetase CSB-YP479626SWK
CSB-EP479626SWK
CSB-BP479626SWK
CSB-MP479626SWK
CSB-EP479626SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP479627SWK
CSB-EP479627SWK
CSB-BP479627SWK
CSB-MP479627SWK
CSB-EP479627SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA-binding protein fis (fis) CSB-YP479628SWK
CSB-EP479628SWK
CSB-BP479628SWK
CSB-MP479628SWK
CSB-EP479628SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 30S ribosomal protein S4 (rpsD) CSB-YP479629SWK
CSB-EP479629SWK
CSB-BP479629SWK
CSB-MP479629SWK
CSB-EP479629SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L24 (rplX) CSB-YP479630SWK
CSB-EP479630SWK
CSB-BP479630SWK
CSB-MP479630SWK
CSB-EP479630SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 2-isopropylmalate synthase (leuA), partial CSB-YP479631SWK
CSB-EP479631SWK
CSB-BP479631SWK
CSB-MP479631SWK
CSB-EP479631SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA gyrase inhibitor YacG (yacG) CSB-YP479632SWK
CSB-EP479632SWK
CSB-BP479632SWK
CSB-MP479632SWK
CSB-EP479632SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Aspartate 1-decarboxylase (panD) CSB-YP479633SWK
CSB-EP479633SWK
CSB-BP479633SWK
CSB-MP479633SWK
CSB-EP479633SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Peptidase T (pepT) CSB-YP479634SWK
CSB-EP479634SWK
CSB-BP479634SWK
CSB-MP479634SWK
CSB-EP479634SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 30S ribosomal protein S19 (rpsS) CSB-YP479635SWK
CSB-EP479635SWK
CSB-BP479635SWK
CSB-MP479635SWK
CSB-EP479635SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein TusC (tusC) CSB-YP479636SWK
CSB-EP479636SWK
CSB-BP479636SWK
CSB-MP479636SWK
CSB-EP479636SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Shikimate kinase 1 (aroK) CSB-YP479637SWK
CSB-EP479637SWK
CSB-BP479637SWK
CSB-MP479637SWK
CSB-EP479637SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP479638SWK
CSB-EP479638SWK
CSB-BP479638SWK
CSB-MP479638SWK
CSB-EP479638SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribonuclease HII (rnhB) CSB-YP479639SWK
CSB-EP479639SWK
CSB-BP479639SWK
CSB-MP479639SWK
CSB-EP479639SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona O-acetyl-ADP-ribose deacetylase (ymdB) CSB-YP479640SWK
CSB-EP479640SWK
CSB-BP479640SWK
CSB-MP479640SWK
CSB-EP479640SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cytoplasmic trehalase (treF), partial CSB-YP479641SWK
CSB-EP479641SWK
CSB-BP479641SWK
CSB-MP479641SWK
CSB-EP479641SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0301 protein VFMJ11_0434 (VFMJ11_0434) CSB-YP479642VFC
CSB-EP479642VFC
CSB-BP479642VFC
CSB-MP479642VFC
CSB-EP479642VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri [Protein-PII] uridylyltransferase (glnD), partial CSB-YP479643VFC
CSB-EP479643VFC
CSB-BP479643VFC
CSB-MP479643VFC
CSB-EP479643VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Protein grpE (grpE) CSB-YP479644VFC
CSB-EP479644VFC
CSB-BP479644VFC
CSB-MP479644VFC
CSB-EP479644VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S15 (rpsO) CSB-YP479645VFC
CSB-EP479645VFC
CSB-BP479645VFC
CSB-MP479645VFC
CSB-EP479645VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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