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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae serotype 19F CCA-adding enzyme (cca) CSB-YP479499FNE
CSB-EP479499FNE
CSB-BP479499FNE
CSB-MP479499FNE
CSB-EP479499FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Probable GTP-binding protein EngB (engB) CSB-YP479500FNE
CSB-EP479500FNE
CSB-BP479500FNE
CSB-MP479500FNE
CSB-EP479500FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP479501FNE
CSB-EP479501FNE
CSB-BP479501FNE
CSB-MP479501FNE
CSB-EP479501FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F UPF0398 protein SPG_0336 (SPG_0336) CSB-YP479502FNE
CSB-EP479502FNE
CSB-BP479502FNE
CSB-MP479502FNE
CSB-EP479502FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Tryptophan synthase alpha chain (trpA) CSB-YP479503FNE
CSB-EP479503FNE
CSB-BP479503FNE
CSB-MP479503FNE
CSB-EP479503FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP479504FNE
CSB-EP479504FNE
CSB-BP479504FNE
CSB-MP479504FNE
CSB-EP479504FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Pantothenate synthetase (panC) CSB-YP479505GFN
CSB-EP479505GFN
CSB-BP479505GFN
CSB-MP479505GFN
CSB-EP479505GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Serine--tRNA ligase (serS) CSB-YP479506GFN
CSB-EP479506GFN
CSB-BP479506GFN
CSB-MP479506GFN
CSB-EP479506GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 3-dehydroquinate synthase (aroB) CSB-YP479507GFN
CSB-EP479507GFN
CSB-BP479507GFN
CSB-MP479507GFN
CSB-EP479507GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis NADH-quinone oxidoreductase subunit A 1 (nuoA1), partial CSB-YP479508GFN1
CSB-EP479508GFN1
CSB-BP479508GFN1
CSB-MP479508GFN1
CSB-EP479508GFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L28 (rpmB) CSB-YP479509GFN
CSB-EP479509GFN
CSB-BP479509GFN
CSB-MP479509GFN
CSB-EP479509GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Cytidylate kinase (cmk) CSB-YP479510GFN
CSB-EP479510GFN
CSB-BP479510GFN
CSB-MP479510GFN
CSB-EP479510GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP479511GFN
CSB-EP479511GFN
CSB-BP479511GFN
CSB-MP479511GFN
CSB-EP479511GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Selenide, water dikinase (selD) CSB-YP479512GFN
CSB-EP479512GFN
CSB-BP479512GFN
CSB-MP479512GFN
CSB-EP479512GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP479513GFN
CSB-EP479513GFN
CSB-BP479513GFN
CSB-MP479513GFN
CSB-EP479513GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Threonine--tRNA ligase (thrS), partial CSB-YP479514GFN
CSB-EP479514GFN
CSB-BP479514GFN
CSB-MP479514GFN
CSB-EP479514GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Putative nickel-responsive regulator (Gbem_3562) CSB-YP479515GFN
CSB-EP479515GFN
CSB-BP479515GFN
CSB-MP479515GFN
CSB-EP479515GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UPF0272 protein Gbem_3626 (Gbem_3626) CSB-YP479516GFN
CSB-EP479516GFN
CSB-BP479516GFN
CSB-MP479516GFN
CSB-EP479516GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP479517GFN
CSB-EP479517GFN
CSB-BP479517GFN
CSB-MP479517GFN
CSB-EP479517GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis GTP cyclohydrolase folE2 (folE2) CSB-YP479518GFN
CSB-EP479518GFN
CSB-BP479518GFN
CSB-MP479518GFN
CSB-EP479518GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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