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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella agona Ribonuclease HII (rnhB) CSB-YP479639SWK
CSB-EP479639SWK
CSB-BP479639SWK
CSB-MP479639SWK
CSB-EP479639SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona O-acetyl-ADP-ribose deacetylase (ymdB) CSB-YP479640SWK
CSB-EP479640SWK
CSB-BP479640SWK
CSB-MP479640SWK
CSB-EP479640SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cytoplasmic trehalase (treF), partial CSB-YP479641SWK
CSB-EP479641SWK
CSB-BP479641SWK
CSB-MP479641SWK
CSB-EP479641SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0301 protein VFMJ11_0434 (VFMJ11_0434) CSB-YP479642VFC
CSB-EP479642VFC
CSB-BP479642VFC
CSB-MP479642VFC
CSB-EP479642VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri [Protein-PII] uridylyltransferase (glnD), partial CSB-YP479643VFC
CSB-EP479643VFC
CSB-BP479643VFC
CSB-MP479643VFC
CSB-EP479643VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Protein grpE (grpE) CSB-YP479644VFC
CSB-EP479644VFC
CSB-BP479644VFC
CSB-MP479644VFC
CSB-EP479644VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S15 (rpsO) CSB-YP479645VFC
CSB-EP479645VFC
CSB-BP479645VFC
CSB-MP479645VFC
CSB-EP479645VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L19 (rplS) CSB-YP479646VFC
CSB-EP479646VFC
CSB-BP479646VFC
CSB-MP479646VFC
CSB-EP479646VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri DNA repair protein recO (recO) CSB-YP479647VFC
CSB-EP479647VFC
CSB-BP479647VFC
CSB-MP479647VFC
CSB-EP479647VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribose-5-phosphate isomerase A (rpiA) CSB-YP479648VFC
CSB-EP479648VFC
CSB-BP479648VFC
CSB-MP479648VFC
CSB-EP479648VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP479649VFC
CSB-EP479649VFC
CSB-BP479649VFC
CSB-MP479649VFC
CSB-EP479649VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0178 protein VFMJ11_0615 (VFMJ11_0615) CSB-YP479650VFC
CSB-EP479650VFC
CSB-BP479650VFC
CSB-MP479650VFC
CSB-EP479650VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri DNA gyrase inhibitor YacG (yacG) CSB-YP479651VFC
CSB-EP479651VFC
CSB-BP479651VFC
CSB-MP479651VFC
CSB-EP479651VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP479652VFC
CSB-EP479652VFC
CSB-BP479652VFC
CSB-MP479652VFC
CSB-EP479652VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0253 protein VFMJ11_0680 (VFMJ11_0680) CSB-YP479653VFC
CSB-EP479653VFC
CSB-BP479653VFC
CSB-MP479653VFC
CSB-EP479653VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Urease accessory protein UreE (ureE) CSB-YP479654VFC
CSB-EP479654VFC
CSB-BP479654VFC
CSB-MP479654VFC
CSB-EP479654VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Urease subunit gamma (ureA) CSB-YP479655VFC
CSB-EP479655VFC
CSB-BP479655VFC
CSB-MP479655VFC
CSB-EP479655VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Na (+)-translocating NADH-quinone reductase subunit D, partial CSB-YP479656VFC1
CSB-EP479656VFC1
CSB-BP479656VFC1
CSB-MP479656VFC1
CSB-EP479656VFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Probable rRNA maturation factor (VFMJ11_0776) CSB-YP479657VFC
CSB-EP479657VFC
CSB-BP479657VFC
CSB-MP479657VFC
CSB-EP479657VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Shikimate kinase (aroK) CSB-YP479658VFC
CSB-EP479658VFC
CSB-BP479658VFC
CSB-MP479658VFC
CSB-EP479658VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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