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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella agona Phosphoheptose isomerase (gmhA) CSB-YP479559SWK
CSB-EP479559SWK
CSB-BP479559SWK
CSB-MP479559SWK
CSB-EP479559SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Shikimate kinase 2 (aroL) CSB-YP479560SWK
CSB-EP479560SWK
CSB-BP479560SWK
CSB-MP479560SWK
CSB-EP479560SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cobalt transport protein CbiN (cbiN), partial CSB-YP479561SWK1
CSB-EP479561SWK1
CSB-BP479561SWK1
CSB-MP479561SWK1
CSB-EP479561SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP479562SWK
CSB-EP479562SWK
CSB-BP479562SWK
CSB-MP479562SWK
CSB-EP479562SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP479563SWK
CSB-EP479563SWK
CSB-BP479563SWK
CSB-MP479563SWK
CSB-EP479563SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L33 (rpmG) CSB-YP479564SWK
CSB-EP479564SWK
CSB-BP479564SWK
CSB-MP479564SWK
CSB-EP479564SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA ligase B (ligB), partial CSB-YP479565SWK
CSB-EP479565SWK
CSB-BP479565SWK
CSB-MP479565SWK
CSB-EP479565SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona tRNA sulfurtransferase (thiI) CSB-YP479566SWK
CSB-EP479566SWK
CSB-BP479566SWK
CSB-MP479566SWK
CSB-EP479566SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Recombination protein RecR (recR) CSB-YP479567SWK
CSB-EP479567SWK
CSB-BP479567SWK
CSB-MP479567SWK
CSB-EP479567SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP479568SWK
CSB-EP479568SWK
CSB-BP479568SWK
CSB-MP479568SWK
CSB-EP479568SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Small heat shock protein ibpB (ibpB) CSB-YP479569SWK
CSB-EP479569SWK
CSB-BP479569SWK
CSB-MP479569SWK
CSB-EP479569SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cysteine--tRNA ligase (cysS) CSB-YP479570SWK
CSB-EP479570SWK
CSB-BP479570SWK
CSB-MP479570SWK
CSB-EP479570SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0352 protein yejL (yejL) CSB-YP479571SWK
CSB-EP479571SWK
CSB-BP479571SWK
CSB-MP479571SWK
CSB-EP479571SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Bifunctional protein GlmU (glmU) CSB-YP479572SWK
CSB-EP479572SWK
CSB-BP479572SWK
CSB-MP479572SWK
CSB-EP479572SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein viaA (viaA) CSB-YP479573SWK
CSB-EP479573SWK
CSB-BP479573SWK
CSB-MP479573SWK
CSB-EP479573SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Dihydroxy-acid dehydratase (ilvD), partial CSB-YP479574SWK
CSB-EP479574SWK
CSB-BP479574SWK
CSB-MP479574SWK
CSB-EP479574SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Leucine--tRNA ligase (leuS), partial CSB-YP479575SWK
CSB-EP479575SWK
CSB-BP479575SWK
CSB-MP479575SWK
CSB-EP479575SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glutamine--tRNA ligase (glnS) CSB-YP479576SWK
CSB-EP479576SWK
CSB-BP479576SWK
CSB-MP479576SWK
CSB-EP479576SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) CSB-YP479577SWK
CSB-EP479577SWK
CSB-BP479577SWK
CSB-MP479577SWK
CSB-EP479577SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP479578SWK
CSB-EP479578SWK
CSB-BP479578SWK
CSB-MP479578SWK
CSB-EP479578SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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