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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin UPF0434 protein ycaR (ycaR) CSB-YP479739SWN
CSB-EP479739SWN
CSB-BP479739SWN
CSB-MP479739SWN
CSB-EP479739SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP479740SWN
CSB-EP479740SWN
CSB-BP479740SWN
CSB-MP479740SWN
CSB-EP479740SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Phosphomethylpyrimidine synthase (thiC), partial CSB-YP479741SWN
CSB-EP479741SWN
CSB-BP479741SWN
CSB-MP479741SWN
CSB-EP479741SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP479742SWN
CSB-EP479742SWN
CSB-BP479742SWN
CSB-MP479742SWN
CSB-EP479742SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Curved DNA-binding protein (cbpA) CSB-YP479743SWN
CSB-EP479743SWN
CSB-BP479743SWN
CSB-MP479743SWN
CSB-EP479743SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Fumarate reductase subunit D (frdD), partial CSB-YP479744SWN1
CSB-EP479744SWN1
CSB-BP479744SWN1
CSB-MP479744SWN1
CSB-EP479744SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L19 (rplS) CSB-YP479745SWN
CSB-EP479745SWN
CSB-BP479745SWN
CSB-MP479745SWN
CSB-EP479745SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 30S ribosomal protein S18 (rpsR) CSB-YP479746SWN
CSB-EP479746SWN
CSB-BP479746SWN
CSB-MP479746SWN
CSB-EP479746SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Probable cytosol aminopeptidase (pepA), partial CSB-YP479747SWN
CSB-EP479747SWN
CSB-BP479747SWN
CSB-MP479747SWN
CSB-EP479747SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP479748SWN
CSB-EP479748SWN
CSB-BP479748SWN
CSB-MP479748SWN
CSB-EP479748SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein nrdI (nrdI) CSB-YP479749SWN
CSB-EP479749SWN
CSB-BP479749SWN
CSB-MP479749SWN
CSB-EP479749SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP479750SWN
CSB-EP479750SWN
CSB-BP479750SWN
CSB-MP479750SWN
CSB-EP479750SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP479751SWN
CSB-EP479751SWN
CSB-BP479751SWN
CSB-MP479751SWN
CSB-EP479751SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Lipoate-protein ligase A (lplA) CSB-YP479752SWN
CSB-EP479752SWN
CSB-BP479752SWN
CSB-MP479752SWN
CSB-EP479752SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin ProP effector (proQ) CSB-YP479753SWN
CSB-EP479753SWN
CSB-BP479753SWN
CSB-MP479753SWN
CSB-EP479753SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP479754SWN
CSB-EP479754SWN
CSB-BP479754SWN
CSB-MP479754SWN
CSB-EP479754SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP479755SWN
CSB-EP479755SWN
CSB-BP479755SWN
CSB-MP479755SWN
CSB-EP479755SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Adenylyl-sulfate kinase (cysC) CSB-YP479756SWN
CSB-EP479756SWN
CSB-BP479756SWN
CSB-MP479756SWN
CSB-EP479756SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP479757SWN
CSB-EP479757SWN
CSB-BP479757SWN
CSB-MP479757SWN
CSB-EP479757SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin tRNA-modifying protein ygfZ (ygfZ) CSB-YP479758SWN
CSB-EP479758SWN
CSB-BP479758SWN
CSB-MP479758SWN
CSB-EP479758SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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