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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella dublin Adenylosuccinate synthetase (purA) CSB-YP480739SWN
CSB-EP480739SWN
CSB-BP480739SWN
CSB-MP480739SWN
CSB-EP480739SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 30S ribosomal protein S6 (rpsF) CSB-YP480740SWN
CSB-EP480740SWN
CSB-BP480740SWN
CSB-MP480740SWN
CSB-EP480740SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L9 (rplI) CSB-YP480741SWN
CSB-EP480741SWN
CSB-BP480741SWN
CSB-MP480741SWN
CSB-EP480741SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin tRNA (mo5U34)-methyltransferase CSB-YP480742SWN
CSB-EP480742SWN
CSB-BP480742SWN
CSB-MP480742SWN
CSB-EP480742SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Trp operon repressor (trpR) CSB-YP480743SWN
CSB-EP480743SWN
CSB-BP480743SWN
CSB-MP480743SWN
CSB-EP480743SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein mgrB (mgrB), partial CSB-YP480744SWN
CSB-EP480744SWN
CSB-BP480744SWN
CSB-MP480744SWN
CSB-EP480744SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin tRNA pseudouridine synthase D (truD) CSB-YP480745SWN
CSB-EP480745SWN
CSB-BP480745SWN
CSB-MP480745SWN
CSB-EP480745SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP480746SWN
CSB-EP480746SWN
CSB-BP480746SWN
CSB-MP480746SWN
CSB-EP480746SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP480747SWN
CSB-EP480747SWN
CSB-BP480747SWN
CSB-MP480747SWN
CSB-EP480747SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Glutamyl-tRNA reductase (hemA) CSB-YP480748SWN
CSB-EP480748SWN
CSB-BP480748SWN
CSB-MP480748SWN
CSB-EP480748SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Tryptophan synthase alpha chain (trpA) CSB-YP480749SWN
CSB-EP480749SWN
CSB-BP480749SWN
CSB-MP480749SWN
CSB-EP480749SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin DNA mismatch repair protein mutH (mutH) CSB-YP480750SWN
CSB-EP480750SWN
CSB-BP480750SWN
CSB-MP480750SWN
CSB-EP480750SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chromosome initiation inhibitor (iciA) CSB-YP480751SWN
CSB-EP480751SWN
CSB-BP480751SWN
CSB-MP480751SWN
CSB-EP480751SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin S-adenosylmethionine synthase (metK) CSB-YP480752SWN
CSB-EP480752SWN
CSB-BP480752SWN
CSB-MP480752SWN
CSB-EP480752SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Putative Holliday junction resolvase (yqgF) CSB-YP480753SWN
CSB-EP480753SWN
CSB-BP480753SWN
CSB-MP480753SWN
CSB-EP480753SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0114 protein YqhA (yqhA), partial CSB-YP480754SWN1
CSB-EP480754SWN1
CSB-BP480754SWN1
CSB-MP480754SWN1
CSB-EP480754SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Otolemur garnettii 39S ribosomal protein L23, mitochondrial (MRPL23) CSB-YP480755OFS
CSB-EP480755OFS
CSB-BP480755OFS
CSB-MP480755OFS
CSB-EP480755OFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Potassium channel toxin alpha-KTx J123 CSB-YP480756MRE
CSB-EP480756MRE
CSB-BP480756MRE
CSB-MP480756MRE
CSB-EP480756MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus microlepidotus Protease inhibitor microlepidin-4 CSB-YP480757OGE
CSB-EP480757OGE
CSB-BP480757OGE
CSB-MP480757OGE
CSB-EP480757OGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhinoplocephalus nigrescens Protease inhibitor nigrescinin-4 CSB-YP480758RKG
CSB-EP480758RKG
CSB-BP480758RKG
CSB-MP480758RKG
CSB-EP480758RKG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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