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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP479759SWN
CSB-EP479759SWN
CSB-BP479759SWN
CSB-MP479759SWN
CSB-EP479759SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0441 protein ygiB (ygiB) CSB-YP479760SWN
CSB-EP479760SWN
CSB-BP479760SWN
CSB-MP479760SWN
CSB-EP479760SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudechis australis Auswaprin-a CSB-YP479761EZS
CSB-EP479761EZS
CSB-BP479761EZS
CSB-MP479761EZS
CSB-EP479761EZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aciduliprofundum boonei Hypoxanthine/guanine phosphoribosyltransferase (hpt) CSB-YP479762AWL
CSB-EP479762AWL
CSB-BP479762AWL
CSB-MP479762AWL
CSB-EP479762AWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notechis scutatus scutatus Protease inhibitor tigerin-3 CSB-YP479763NHT
CSB-EP479763NHT
CSB-BP479763NHT
CSB-MP479763NHT
CSB-EP479763NHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps superbus Protease inhibitor superbin-2 CSB-YP479764DPN
CSB-EP479764DPN
CSB-BP479764DPN
CSB-MP479764DPN
CSB-EP479764DPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudechis porphyriacus Porwaprin-d CSB-YP479765EZT
CSB-EP479765EZT
CSB-BP479765EZT
CSB-MP479765EZT
CSB-EP479765EZT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Demansia vestigiata Veswaprin-c CSB-YP479766DJD
CSB-EP479766DJD
CSB-BP479766DJD
CSB-MP479766DJD
CSB-EP479766DJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus microlepidotus Protease inhibitor microlepidin-5 CSB-YP479767OGE
CSB-EP479767OGE
CSB-BP479767OGE
CSB-MP479767OGE
CSB-EP479767OGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP479768DTI1
CSB-EP479768DTI1
CSB-BP479768DTI1
CSB-MP479768DTI1
CSB-EP479768DTI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP479769DTI1
CSB-EP479769DTI1
CSB-BP479769DTI1
CSB-MP479769DTI1
CSB-EP479769DTI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP479770DTI
CSB-EP479770DTI
CSB-BP479770DTI
CSB-MP479770DTI
CSB-EP479770DTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP479771DTI
CSB-EP479771DTI
CSB-BP479771DTI
CSB-MP479771DTI
CSB-EP479771DTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria fallax Preprofallaxidin-6 CSB-YP479772LQD
CSB-EP479772LQD
CSB-BP479772LQD
CSB-MP479772LQD
CSB-EP479772LQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 N utilization substance protein B homolog (nusB) CSB-YP479773SWO
CSB-EP479773SWO
CSB-BP479773SWO
CSB-MP479773SWO
CSB-EP479773SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP479774SWO
CSB-EP479774SWO
CSB-BP479774SWO
CSB-MP479774SWO
CSB-EP479774SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 D-serine dehydratase (dsdA) CSB-YP479775SWO
CSB-EP479775SWO
CSB-BP479775SWO
CSB-MP479775SWO
CSB-EP479775SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 50S ribosomal protein L31 type B (rpmE2) CSB-YP479776SWO
CSB-EP479776SWO
CSB-BP479776SWO
CSB-MP479776SWO
CSB-EP479776SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 DNA replication terminus site-binding protein (tus) CSB-YP479777SWO
CSB-EP479777SWO
CSB-BP479777SWO
CSB-MP479777SWO
CSB-EP479777SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 30S ribosomal protein S16 (rpsP) CSB-YP479778SWO
CSB-EP479778SWO
CSB-BP479778SWO
CSB-MP479778SWO
CSB-EP479778SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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