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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP481139TMY
CSB-EP481139TMY
CSB-BP481139TMY
CSB-MP481139TMY
CSB-EP481139TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii NADH-quinone oxidoreductase subunit B 1 (nuoB1) CSB-YP481140TMY
CSB-EP481140TMY
CSB-BP481140TMY
CSB-MP481140TMY
CSB-EP481140TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP481141TMY
CSB-EP481141TMY
CSB-BP481141TMY
CSB-MP481141TMY
CSB-EP481141TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP481142TMY
CSB-EP481142TMY
CSB-BP481142TMY
CSB-MP481142TMY
CSB-EP481142TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UPF0133 protein THEYE_A1069 (THEYE_A1069) CSB-YP481143TMY
CSB-EP481143TMY
CSB-BP481143TMY
CSB-MP481143TMY
CSB-EP481143TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP481144TMY
CSB-EP481144TMY
CSB-BP481144TMY
CSB-MP481144TMY
CSB-EP481144TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thalassiosira pseudonana Cyanate hydratase (CYN) CSB-YP481145TLZ
CSB-EP481145TLZ
CSB-BP481145TLZ
CSB-MP481145TLZ
CSB-EP481145TLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Proofreading thioesterase EntH (entH) CSB-YP481146EOE
CSB-EP481146EOE
CSB-BP481146EOE
CSB-MP481146EOE
CSB-EP481146EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-dehydroquinate dehydratase (aroD) CSB-YP481147EOE
CSB-EP481147EOE
CSB-BP481147EOE
CSB-MP481147EOE
CSB-EP481147EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP481148EOE
CSB-EP481148EOE
CSB-BP481148EOE
CSB-MP481148EOE
CSB-EP481148EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Succinylglutamate desuccinylase (astE) CSB-YP481149EOE
CSB-EP481149EOE
CSB-BP481149EOE
CSB-MP481149EOE
CSB-EP481149EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0229 protein yeaH (yeaH) CSB-YP481150EOE
CSB-EP481150EOE
CSB-BP481150EOE
CSB-MP481150EOE
CSB-EP481150EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0149 protein ygfB (ygfB) CSB-YP481151EOE
CSB-EP481151EOE
CSB-BP481151EOE
CSB-MP481151EOE
CSB-EP481151EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Biosynthetic arginine decarboxylase (speA) CSB-YP481152EOE
CSB-EP481152EOE
CSB-BP481152EOE
CSB-MP481152EOE
CSB-EP481152EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0250 protein ybeD (ybeD) CSB-YP481153EOE
CSB-EP481153EOE
CSB-BP481153EOE
CSB-MP481153EOE
CSB-EP481153EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable rRNA maturation factor YbeY (ybeY) CSB-YP481154EOE
CSB-EP481154EOE
CSB-BP481154EOE
CSB-MP481154EOE
CSB-EP481154EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Potassium-transporting ATPase B chain (kdpB), partial CSB-YP481155EOE1
CSB-EP481155EOE1
CSB-BP481155EOE1
CSB-MP481155EOE1
CSB-EP481155EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0756 membrane protein YeaL (yeaL), partial CSB-YP481156EOE1
CSB-EP481156EOE1
CSB-BP481156EOE1
CSB-MP481156EOE1
CSB-EP481156EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0059 membrane protein yebN (yebN), partial CSB-YP481157EOE1
CSB-EP481157EOE1
CSB-BP481157EOE1
CSB-MP481157EOE1
CSB-EP481157EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP481158EOE
CSB-EP481158EOE
CSB-BP481158EOE
CSB-MP481158EOE
CSB-EP481158EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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