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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum [Protein-PII] uridylyltransferase (glnD), partial CSB-YP479939SWP
CSB-EP479939SWP
CSB-BP479939SWP
CSB-MP479939SWP
CSB-EP479939SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 30S ribosomal protein S21 (rpsU) CSB-YP479940BUH
CSB-EP479940BUH
CSB-BP479940BUH
CSB-MP479940BUH
CSB-EP479940BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP479941BUH
CSB-EP479941BUH
CSB-BP479941BUH
CSB-MP479941BUH
CSB-EP479941BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Uridine kinase (udk) CSB-YP479942BUH
CSB-EP479942BUH
CSB-BP479942BUH
CSB-MP479942BUH
CSB-EP479942BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP479943BUH
CSB-EP479943BUH
CSB-BP479943BUH
CSB-MP479943BUH
CSB-EP479943BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L33 (rpmG) CSB-YP479944BUH
CSB-EP479944BUH
CSB-BP479944BUH
CSB-MP479944BUH
CSB-EP479944BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L2 (rplB) CSB-YP479945BUH
CSB-EP479945BUH
CSB-BP479945BUH
CSB-MP479945BUH
CSB-EP479945BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L29 (rpmC) CSB-YP479946BUH
CSB-EP479946BUH
CSB-BP479946BUH
CSB-MP479946BUH
CSB-EP479946BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L36 (rpmJ) CSB-YP479947BUH
CSB-EP479947BUH
CSB-BP479947BUH
CSB-MP479947BUH
CSB-EP479947BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Methionine--tRNA ligase (metG), partial CSB-YP479948BUH
CSB-EP479948BUH
CSB-BP479948BUH
CSB-MP479948BUH
CSB-EP479948BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP479949BUH
CSB-EP479949BUH
CSB-BP479949BUH
CSB-MP479949BUH
CSB-EP479949BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Putative septation protein spoVG (spoVG) CSB-YP479950BUH
CSB-EP479950BUH
CSB-BP479950BUH
CSB-MP479950BUH
CSB-EP479950BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L2 (rplB) CSB-YP479951BUK
CSB-EP479951BUK
CSB-BP479951BUK
CSB-MP479951BUK
CSB-EP479951BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L24 (rplX) CSB-YP479952BUK
CSB-EP479952BUK
CSB-BP479952BUK
CSB-MP479952BUK
CSB-EP479952BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Chaperone protein DnaK (dnaK), partial CSB-YP479953BUK
CSB-EP479953BUK
CSB-BP479953BUK
CSB-MP479953BUK
CSB-EP479953BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Arginine--tRNA ligase (argS), partial CSB-YP479954BUK
CSB-EP479954BUK
CSB-BP479954BUK
CSB-MP479954BUK
CSB-EP479954BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP479955BUK
CSB-EP479955BUK
CSB-BP479955BUK
CSB-MP479955BUK
CSB-EP479955BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L32 (rpmF) CSB-YP479956BUK
CSB-EP479956BUK
CSB-BP479956BUK
CSB-MP479956BUK
CSB-EP479956BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Putative septation protein spoVG (spoVG) CSB-YP479957BUK
CSB-EP479957BUK
CSB-BP479957BUK
CSB-MP479957BUK
CSB-EP479957BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP479958BUK
CSB-EP479958BUK
CSB-BP479958BUK
CSB-MP479958BUK
CSB-EP479958BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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