Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Borrelia recurrentis V-type ATP synthase alpha chain (atpA), partial CSB-YP479959BUK
CSB-EP479959BUK
CSB-BP479959BUK
CSB-MP479959BUK
CSB-EP479959BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L20 (rplT) CSB-YP479960BUK
CSB-EP479960BUK
CSB-BP479960BUK
CSB-MP479960BUK
CSB-EP479960BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Enolase (eno) CSB-YP479961BUK
CSB-EP479961BUK
CSB-BP479961BUK
CSB-MP479961BUK
CSB-EP479961BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L10 (rplJ) CSB-YP479962BUK
CSB-EP479962BUK
CSB-BP479962BUK
CSB-MP479962BUK
CSB-EP479962BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP479963BUK
CSB-EP479963BUK
CSB-BP479963BUK
CSB-MP479963BUK
CSB-EP479963BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Protein SUS1 (SUS1) CSB-YP479964YAP
CSB-EP479964YAP
CSB-BP479964YAP
CSB-MP479964YAP
CSB-EP479964YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ribosome-releasing factor 2, mitochondrial (MEF2), partial CSB-YP479965DIS
CSB-EP479965DIS
CSB-BP479965DIS
CSB-MP479965DIS
CSB-EP479965DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae SWI5-dependent HO expression protein 3 (SHE3) CSB-YP479966SVH
CSB-EP479966SVH
CSB-BP479966SVH
CSB-MP479966SVH
CSB-EP479966SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Required for respiratory growth protein 1, mitochondrial (RRG1) CSB-YP479967SVH
CSB-EP479967SVH
CSB-BP479967SVH
CSB-MP479967SVH
CSB-EP479967SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription factor STP1 (STP1), partial CSB-YP479968SVH
CSB-EP479968SVH
CSB-BP479968SVH
CSB-MP479968SVH
CSB-EP479968SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 30S ribosomal protein S10 (rpsJ1) CSB-YP479969AZX
CSB-EP479969AZX
CSB-BP479969AZX
CSB-MP479969AZX
CSB-EP479969AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 30S ribosomal protein S6 (rpsF) CSB-YP479970AZX
CSB-EP479970AZX
CSB-BP479970AZX
CSB-MP479970AZX
CSB-EP479970AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Shikimate kinase (aroK) CSB-YP479971AZX
CSB-EP479971AZX
CSB-BP479971AZX
CSB-MP479971AZX
CSB-EP479971AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP479972AZX
CSB-EP479972AZX
CSB-BP479972AZX
CSB-MP479972AZX
CSB-EP479972AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribosomal protein L11 methyltransferase (prmA) CSB-YP479973AZX
CSB-EP479973AZX
CSB-BP479973AZX
CSB-MP479973AZX
CSB-EP479973AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP479974AZX
CSB-EP479974AZX
CSB-BP479974AZX
CSB-MP479974AZX
CSB-EP479974AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Probable GTP-binding protein EngB (engB) CSB-YP479975AZX
CSB-EP479975AZX
CSB-BP479975AZX
CSB-MP479975AZX
CSB-EP479975AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Thiazole synthase (thiG) CSB-YP479976AZX
CSB-EP479976AZX
CSB-BP479976AZX
CSB-MP479976AZX
CSB-EP479976AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP479977EYX
CSB-EP479977EYX
CSB-BP479977EYX
CSB-MP479977EYX
CSB-EP479977EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP479978EYX
CSB-EP479978EYX
CSB-BP479978EYX
CSB-MP479978EYX
CSB-EP479978EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>