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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prosthecochloris aestuarii Chaperone protein DnaJ (dnaJ) CSB-YP479999EYX
CSB-EP479999EYX
CSB-BP479999EYX
CSB-MP479999EYX
CSB-EP479999EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP480000EYX
CSB-EP480000EYX
CSB-BP480000EYX
CSB-MP480000EYX
CSB-EP480000EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Adenylate kinase (adk) CSB-YP480001ETB
CSB-EP480001ETB
CSB-BP480001ETB
CSB-MP480001ETB
CSB-EP480001ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme UPF0761 membrane protein Ppha_1623 (Ppha_1623) CSB-YP480002ETB
CSB-EP480002ETB
CSB-BP480002ETB
CSB-MP480002ETB
CSB-EP480002ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L35 (rpmI) CSB-YP480003ETB
CSB-EP480003ETB
CSB-BP480003ETB
CSB-MP480003ETB
CSB-EP480003ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L32 (rpmF) CSB-YP480004ETB
CSB-EP480004ETB
CSB-BP480004ETB
CSB-MP480004ETB
CSB-EP480004ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 30S ribosomal protein S10 (rpsJ) CSB-YP480005ETB
CSB-EP480005ETB
CSB-BP480005ETB
CSB-MP480005ETB
CSB-EP480005ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 30S ribosomal protein S19 (rpsS) CSB-YP480006ETB
CSB-EP480006ETB
CSB-BP480006ETB
CSB-MP480006ETB
CSB-EP480006ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L18 (rplR) CSB-YP480007ETB
CSB-EP480007ETB
CSB-BP480007ETB
CSB-MP480007ETB
CSB-EP480007ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP480008ETB
CSB-EP480008ETB
CSB-BP480008ETB
CSB-MP480008ETB
CSB-EP480008ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP480009ETB
CSB-EP480009ETB
CSB-BP480009ETB
CSB-MP480009ETB
CSB-EP480009ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP480010ETB
CSB-EP480010ETB
CSB-BP480010ETB
CSB-MP480010ETB
CSB-EP480010ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP480011ETB
CSB-EP480011ETB
CSB-BP480011ETB
CSB-MP480011ETB
CSB-EP480011ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP480012ETB
CSB-EP480012ETB
CSB-BP480012ETB
CSB-MP480012ETB
CSB-EP480012ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme HPr kinase/phosphorylase (hprK) CSB-YP480013ETB
CSB-EP480013ETB
CSB-BP480013ETB
CSB-MP480013ETB
CSB-EP480013ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L28 (rpmB) CSB-YP480014ETB
CSB-EP480014ETB
CSB-BP480014ETB
CSB-MP480014ETB
CSB-EP480014ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 30S ribosomal protein S9 (rpsI) CSB-YP480015ETB
CSB-EP480015ETB
CSB-BP480015ETB
CSB-MP480015ETB
CSB-EP480015ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 10 kDa chaperonin (groS) CSB-YP480016ETB
CSB-EP480016ETB
CSB-BP480016ETB
CSB-MP480016ETB
CSB-EP480016ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP480017ETB
CSB-EP480017ETB
CSB-BP480017ETB
CSB-MP480017ETB
CSB-EP480017ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Dihydrodipicolinate reductase (dapB) CSB-YP480018ETB
CSB-EP480018ETB
CSB-BP480018ETB
CSB-MP480018ETB
CSB-EP480018ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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