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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Arachidonate 5-lipoxygenase (ALOX5) CSB-YP001624HU
CSB-BP001624HU
CSB-MP001624HU
CSB-EP001624HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Granulocyte colony-stimulating factor (CSF3) CSB-YP006049HU
CSB-EP006049HU
CSB-BP006049HU
CSB-MP006049HU
CSB-EP006049HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Surfeit locus protein 1 (Surf1), partial CSB-YP022963MO1
CSB-EP022963MO1
CSB-BP022963MO1
CSB-MP022963MO1
CSB-EP022963MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Thyroid peroxidase (TPO), partial CSB-YP024112PI
CSB-EP024112PI
CSB-BP024112PI
CSB-MP024112PI
CSB-EP024112PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Synapsin-1 (Syn1) CSB-YP023002RA
CSB-EP023002RA
CSB-BP023002RA
CSB-MP023002RA
CSB-EP023002RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Carboxypeptidase B (CPB1) CSB-YP005883PI
CSB-EP005883PI
CSB-BP005883PI
CSB-MP005883PI
CSB-EP005883PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Fructose-bisphosphate aldolase C (ALDOC) CSB-MP001587HU
CSB-EP001587HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome c1, heme protein, mitochondrial (CYC1), partial CSB-YP006327BO1
CSB-EP006327BO1
CSB-BP006327BO1
CSB-MP006327BO1
CSB-EP006327BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome b-c1 complex subunit 7 (UQCRB) CSB-YP025664BO
CSB-EP025664BO
CSB-BP025664BO
CSB-MP025664BO
CSB-EP025664BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome b5 (CYB5A), partial CSB-YP006309BO1
CSB-EP006309BO1
CSB-BP006309BO1
CSB-MP006309BO1
CSB-EP006309BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) CSB-YP006389BO
CSB-EP006389BO
CSB-BP006389BO
CSB-MP006389BO
CSB-EP006389BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human L-lactate dehydrogenase A chain (LDHA) CSB-BP012832HU
CSB-MP012832HU
CSB-EP012832HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glutamate dehydrogenase 1, mitochondrial (GLUD1) CSB-YP009544HU
CSB-EP009544HU
CSB-BP009544HU
CSB-MP009544HU
CSB-EP009544HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Dihydrofolate reductase (Dhfr) CSB-YP006847MO
CSB-EP006847MO
CSB-BP006847MO
CSB-MP006847MO
CSB-EP006847MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH-cytochrome b5 reductase 3 (CYB5R3), partial CSB-YP006320HU
CSB-EP006320HU
CSB-BP006320HU
CSB-MP006320HU
CSB-EP006320HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072BO
CSB-EP015072BO
CSB-BP015072BO
CSB-MP015072BO
CSB-EP015072BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074BO1
CSB-EP015074BO1
CSB-BP015074BO1
CSB-MP015074BO1
CSB-EP015074BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1), partial CSB-YP005832BO1
CSB-EP005832BO1
CSB-BP005832BO1
CSB-MP005832BO1
CSB-EP005832BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Catalase (CAT), partial CSB-YP004563BO
CSB-EP004563BO
CSB-BP004563BO
CSB-MP004563BO
CSB-EP004563BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP022397HU
CSB-EP022397HU
CSB-BP022397HU
CSB-MP022397HU
CSB-EP022397HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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