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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Transmembrane protein 115 (TMEM115), partial CSB-YP023683BO1
CSB-EP023683BO1
CSB-BP023683BO1
CSB-MP023683BO1
CSB-EP023683BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 116 kDa U5 small nuclear ribonucleoprotein component (EFTUD2), partial CSB-YP007472BO
CSB-EP007472BO
CSB-BP007472BO
CSB-MP007472BO
CSB-EP007472BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine DNA replication licensing factor MCM3 (MCM3), partial CSB-YP013591BO
CSB-EP013591BO
CSB-BP013591BO
CSB-MP013591BO
CSB-EP013591BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 60S ribosomal protein L22-like 1 (RPL22L1) CSB-YP020183BO
CSB-EP020183BO
CSB-BP020183BO
CSB-MP020183BO
CSB-EP020183BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Coiled-coil domain-containing protein 58 (CCDC58) CSB-YP004717BO
CSB-EP004717BO
CSB-BP004717BO
CSB-MP004717BO
CSB-EP004717BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Actin-like protein 6B (ACTL6B) CSB-YP001237BO
CSB-EP001237BO
CSB-BP001237BO
CSB-MP001237BO
CSB-EP001237BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Keratin, type II cuticular Hb3 (KRT83) CSB-YP012578BO
CSB-EP012578BO
CSB-BP012578BO
CSB-MP012578BO
CSB-EP012578BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein mab-21-like 1 (MAB21L1) CSB-YP013296BO
CSB-EP013296BO
CSB-BP013296BO
CSB-MP013296BO
CSB-EP013296BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein kinase C delta-binding protein (PRKCDBP) CSB-YP018703BO
CSB-EP018703BO
CSB-BP018703BO
CSB-MP018703BO
CSB-EP018703BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Ras-related protein Rab-8A (RAB8A) CSB-YP019222BO
CSB-EP019222BO
CSB-BP019222BO
CSB-MP019222BO
CSB-EP019222BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cytosolic Fe-S cluster assembly factor NARFL (NARFL) CSB-YP015455BO
CSB-EP015455BO
CSB-BP015455BO
CSB-MP015455BO
CSB-EP015455BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine mRNA turnover protein 4 homolog (MRTO4) CSB-YP014945BO
CSB-EP014945BO
CSB-BP014945BO
CSB-MP014945BO
CSB-EP014945BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human EF-hand calcium-binding domain-containing protein 5 (EFCAB5), partial CSB-YP007445HU
CSB-EP007445HU
CSB-BP007445HU
CSB-MP007445HU
CSB-EP007445HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Gypsy retrotransposon integrase-like protein 1 (GIN1) CSB-YP009428BO
CSB-EP009428BO
CSB-BP009428BO
CSB-MP009428BO
CSB-EP009428BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Rhophilin-2 (RHPN2), partial CSB-YP019704BO
CSB-EP019704BO
CSB-BP019704BO
CSB-MP019704BO
CSB-EP019704BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein FAM164A (FAM164A) CSB-YP008115BO
CSB-EP008115BO
CSB-BP008115BO
CSB-MP008115BO
CSB-EP008115BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Rab GTPase-binding effector protein 2 (RABEP2), partial CSB-YP019229BO
CSB-EP019229BO
CSB-BP019229BO
CSB-MP019229BO
CSB-EP019229BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 (NDUFAF4) CSB-YP015640BO
CSB-EP015640BO
CSB-BP015640BO
CSB-MP015640BO
CSB-EP015640BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Molybdopterin synthase catalytic subunit (MOCS2) CSB-YP014706BO
CSB-EP014706BO
CSB-BP014706BO
CSB-MP014706BO
CSB-EP014706BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Hydroxysteroid dehydrogenase-like protein 2 (HSDL2) CSB-YP010790BO
CSB-EP010790BO
CSB-BP010790BO
CSB-MP010790BO
CSB-EP010790BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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