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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Borrelia duttonii 30S ribosomal protein S17 (rpsQ) CSB-YP480919BUH
CSB-EP480919BUH
CSB-BP480919BUH
CSB-MP480919BUH
CSB-EP480919BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L6 (rplF) CSB-YP480920BUH
CSB-EP480920BUH
CSB-BP480920BUH
CSB-MP480920BUH
CSB-EP480920BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP480921BUH
CSB-EP480921BUH
CSB-BP480921BUH
CSB-MP480921BUH
CSB-EP480921BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 30S ribosomal protein S4 (rpsD) CSB-YP480922BUH
CSB-EP480922BUH
CSB-BP480922BUH
CSB-MP480922BUH
CSB-EP480922BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP480923BUH
CSB-EP480923BUH
CSB-BP480923BUH
CSB-MP480923BUH
CSB-EP480923BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Threonine--tRNA ligase (thrS), partial CSB-YP480924BUH
CSB-EP480924BUH
CSB-BP480924BUH
CSB-MP480924BUH
CSB-EP480924BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Ribosome maturation factor RimP (rimP) CSB-YP480925BUH
CSB-EP480925BUH
CSB-BP480925BUH
CSB-MP480925BUH
CSB-EP480925BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Arginine deiminase (arcA) CSB-YP480926BUH
CSB-EP480926BUH
CSB-BP480926BUH
CSB-MP480926BUH
CSB-EP480926BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 30S ribosomal protein S3 (rpsC) CSB-YP480927BUK
CSB-EP480927BUK
CSB-BP480927BUK
CSB-MP480927BUK
CSB-EP480927BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP480928BUK
CSB-EP480928BUK
CSB-BP480928BUK
CSB-MP480928BUK
CSB-EP480928BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L17 (rplQ) CSB-YP480929BUK
CSB-EP480929BUK
CSB-BP480929BUK
CSB-MP480929BUK
CSB-EP480929BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Methionine--tRNA ligase (metG), partial CSB-YP480930BUK
CSB-EP480930BUK
CSB-BP480930BUK
CSB-MP480930BUK
CSB-EP480930BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 60 kDa chaperonin (groL), partial CSB-YP480931BUK
CSB-EP480931BUK
CSB-BP480931BUK
CSB-MP480931BUK
CSB-EP480931BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis GTPase obg (obg) CSB-YP480932BUK
CSB-EP480932BUK
CSB-BP480932BUK
CSB-MP480932BUK
CSB-EP480932BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Isoleucine--tRNA ligase (ileS), partial CSB-YP480933BUK
CSB-EP480933BUK
CSB-BP480933BUK
CSB-MP480933BUK
CSB-EP480933BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Probable transcriptional regulatory protein BRE_29 (BRE_29) CSB-YP480934BUK
CSB-EP480934BUK
CSB-BP480934BUK
CSB-MP480934BUK
CSB-EP480934BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 30S ribosomal protein S6 (rpsF) CSB-YP480935BUK
CSB-EP480935BUK
CSB-BP480935BUK
CSB-MP480935BUK
CSB-EP480935BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis tRNA modification GTPase MnmE (mnmE) CSB-YP480936BUK
CSB-EP480936BUK
CSB-BP480936BUK
CSB-MP480936BUK
CSB-EP480936BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Elongation factor P (efp) CSB-YP480937BUK
CSB-EP480937BUK
CSB-BP480937BUK
CSB-MP480937BUK
CSB-EP480937BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP480938BUK
CSB-EP480938BUK
CSB-BP480938BUK
CSB-MP480938BUK
CSB-EP480938BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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