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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae Nucleoside triphosphatase nudI (nudI) CSB-YP480999KBH
CSB-EP480999KBH
CSB-BP480999KBH
CSB-MP480999KBH
CSB-EP480999KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable endonuclease 4 (nfo) CSB-YP481000KBH
CSB-EP481000KBH
CSB-BP481000KBH
CSB-MP481000KBH
CSB-EP481000KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Hydroxyethylthiazole kinase (thiM) CSB-YP481001KBH
CSB-EP481001KBH
CSB-BP481001KBH
CSB-MP481001KBH
CSB-EP481001KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Histidinol-phosphate aminotransferase (hisC) CSB-YP481002KBH
CSB-EP481002KBH
CSB-BP481002KBH
CSB-MP481002KBH
CSB-EP481002KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein dsrB (dsrB) CSB-YP481003KBH
CSB-EP481003KBH
CSB-BP481003KBH
CSB-MP481003KBH
CSB-EP481003KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UvrABC system protein C (uvrC), partial CSB-YP481004KBH
CSB-EP481004KBH
CSB-BP481004KBH
CSB-MP481004KBH
CSB-EP481004KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP481005KBH
CSB-EP481005KBH
CSB-BP481005KBH
CSB-MP481005KBH
CSB-EP481005KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP481006KBH
CSB-EP481006KBH
CSB-BP481006KBH
CSB-MP481006KBH
CSB-EP481006KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae D-amino acid dehydrogenase small subunit (dadA) CSB-YP481007KBH
CSB-EP481007KBH
CSB-BP481007KBH
CSB-MP481007KBH
CSB-EP481007KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP481008KBH
CSB-EP481008KBH
CSB-BP481008KBH
CSB-MP481008KBH
CSB-EP481008KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Acetaldehyde dehydrogenase (mhpF) CSB-YP481009KBH
CSB-EP481009KBH
CSB-BP481009KBH
CSB-MP481009KBH
CSB-EP481009KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable sugar efflux transporter (sotB), partial CSB-YP481010KBH
CSB-EP481010KBH
CSB-BP481010KBH
CSB-MP481010KBH
CSB-EP481010KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Spermidine export protein MdtI (mdtI), partial CSB-YP481011KBH1
CSB-EP481011KBH1
CSB-BP481011KBH1
CSB-MP481011KBH1
CSB-EP481011KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0509 protein KPK_3153 (KPK_3153) CSB-YP481012KBH
CSB-EP481012KBH
CSB-BP481012KBH
CSB-MP481012KBH
CSB-EP481012KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein AaeX (aaeX), partial CSB-YP481013KBH1
CSB-EP481013KBH1
CSB-BP481013KBH1
CSB-MP481013KBH1
CSB-EP481013KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP481014KBH
CSB-EP481014KBH
CSB-BP481014KBH
CSB-MP481014KBH
CSB-EP481014KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 30S ribosomal protein S15 (rpsO) CSB-YP481015KBH
CSB-EP481015KBH
CSB-BP481015KBH
CSB-MP481015KBH
CSB-EP481015KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Deoxycytidine triphosphate deaminase (dcd) CSB-YP481016KBH
CSB-EP481016KBH
CSB-BP481016KBH
CSB-MP481016KBH
CSB-EP481016KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP481017KBH
CSB-EP481017KBH
CSB-BP481017KBH
CSB-MP481017KBH
CSB-EP481017KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Nucleoid occlusion factor SlmA (slmA) CSB-YP481018KBH
CSB-EP481018KBH
CSB-BP481018KBH
CSB-MP481018KBH
CSB-EP481018KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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