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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L17 (rplQ) CSB-YP481119TMY
CSB-EP481119TMY
CSB-BP481119TMY
CSB-MP481119TMY
CSB-EP481119TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L16 (rplP) CSB-YP481120TMY
CSB-EP481120TMY
CSB-BP481120TMY
CSB-MP481120TMY
CSB-EP481120TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L4 (rplD) CSB-YP481121TMY
CSB-EP481121TMY
CSB-BP481121TMY
CSB-MP481121TMY
CSB-EP481121TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L32 (rpmF) CSB-YP481122TMY
CSB-EP481122TMY
CSB-BP481122TMY
CSB-MP481122TMY
CSB-EP481122TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S2 (rpsB) CSB-YP481123TMY
CSB-EP481123TMY
CSB-BP481123TMY
CSB-MP481123TMY
CSB-EP481123TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Serine--tRNA ligase (serS) CSB-YP481124TMY
CSB-EP481124TMY
CSB-BP481124TMY
CSB-MP481124TMY
CSB-EP481124TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Histidine--tRNA ligase (hisS) CSB-YP481125TMY
CSB-EP481125TMY
CSB-BP481125TMY
CSB-MP481125TMY
CSB-EP481125TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP481126TMY
CSB-EP481126TMY
CSB-BP481126TMY
CSB-MP481126TMY
CSB-EP481126TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP481127TMY
CSB-EP481127TMY
CSB-BP481127TMY
CSB-MP481127TMY
CSB-EP481127TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UPF0042 nucleotide-binding protein THEYE_A0235 (THEYE_A0235) CSB-YP481128TMY
CSB-EP481128TMY
CSB-BP481128TMY
CSB-MP481128TMY
CSB-EP481128TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP481129TMY
CSB-EP481129TMY
CSB-BP481129TMY
CSB-MP481129TMY
CSB-EP481129TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Glutamate--tRNA ligase (gltX) CSB-YP481130TMY
CSB-EP481130TMY
CSB-BP481130TMY
CSB-MP481130TMY
CSB-EP481130TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP481131TMY
CSB-EP481131TMY
CSB-BP481131TMY
CSB-MP481131TMY
CSB-EP481131TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP481132TMY
CSB-EP481132TMY
CSB-BP481132TMY
CSB-MP481132TMY
CSB-EP481132TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii ATP phosphoribosyltransferase (hisG) CSB-YP481133TMY
CSB-EP481133TMY
CSB-BP481133TMY
CSB-MP481133TMY
CSB-EP481133TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Deoxyguanosinetriphosphate triphosphohydrolase-like protein (THEYE_A0539) CSB-YP481134TMY
CSB-EP481134TMY
CSB-BP481134TMY
CSB-MP481134TMY
CSB-EP481134TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP481135TMY
CSB-EP481135TMY
CSB-BP481135TMY
CSB-MP481135TMY
CSB-EP481135TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP481136TMY
CSB-EP481136TMY
CSB-BP481136TMY
CSB-MP481136TMY
CSB-EP481136TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Glutamyl-tRNA reductase (hemA) CSB-YP481137TMY
CSB-EP481137TMY
CSB-BP481137TMY
CSB-MP481137TMY
CSB-EP481137TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP481138TMY
CSB-EP481138TMY
CSB-BP481138TMY
CSB-MP481138TMY
CSB-EP481138TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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