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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 D-ribose pyranase (rbsD) CSB-YP481199EOE
CSB-EP481199EOE
CSB-BP481199EOE
CSB-MP481199EOE
CSB-EP481199EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP481200EOE
CSB-EP481200EOE
CSB-BP481200EOE
CSB-MP481200EOE
CSB-EP481200EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Diaminopimelate epimerase (dapF) CSB-YP481201EOE
CSB-EP481201EOE
CSB-BP481201EOE
CSB-MP481201EOE
CSB-EP481201EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Homoserine kinase (thrB) CSB-YP481202EOE
CSB-EP481202EOE
CSB-BP481202EOE
CSB-MP481202EOE
CSB-EP481202EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S20 (rpsT) CSB-YP481203EOE
CSB-EP481203EOE
CSB-BP481203EOE
CSB-MP481203EOE
CSB-EP481203EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP481204EOE
CSB-EP481204EOE
CSB-BP481204EOE
CSB-MP481204EOE
CSB-EP481204EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0263 protein yciU (yciU) CSB-YP481205EOE
CSB-EP481205EOE
CSB-BP481205EOE
CSB-MP481205EOE
CSB-EP481205EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Rhamnulokinase (rhaB) CSB-YP481206EOE
CSB-EP481206EOE
CSB-BP481206EOE
CSB-MP481206EOE
CSB-EP481206EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP481207EOE
CSB-EP481207EOE
CSB-BP481207EOE
CSB-MP481207EOE
CSB-EP481207EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L31 (rpmE) CSB-YP481208EOE
CSB-EP481208EOE
CSB-BP481208EOE
CSB-MP481208EOE
CSB-EP481208EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP481209EOE
CSB-EP481209EOE
CSB-BP481209EOE
CSB-MP481209EOE
CSB-EP481209EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP481210EOE
CSB-EP481210EOE
CSB-BP481210EOE
CSB-MP481210EOE
CSB-EP481210EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0231 protein yacL (yacL) CSB-YP481211EOE
CSB-EP481211EOE
CSB-BP481211EOE
CSB-MP481211EOE
CSB-EP481211EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Sugar fermentation stimulation protein A (sfsA) CSB-YP481212EOE
CSB-EP481212EOE
CSB-BP481212EOE
CSB-MP481212EOE
CSB-EP481212EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional regulator murR (murR) CSB-YP481213EOE
CSB-EP481213EOE
CSB-BP481213EOE
CSB-MP481213EOE
CSB-EP481213EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP481214EOE
CSB-EP481214EOE
CSB-BP481214EOE
CSB-MP481214EOE
CSB-EP481214EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP481215EOE
CSB-EP481215EOE
CSB-BP481215EOE
CSB-MP481215EOE
CSB-EP481215EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L1 (rplA) CSB-YP481216EOE
CSB-EP481216EOE
CSB-BP481216EOE
CSB-MP481216EOE
CSB-EP481216EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Deoxyguanosinetriphosphate triphosphohydrolase (dgt) CSB-YP481217EOE
CSB-EP481217EOE
CSB-BP481217EOE
CSB-MP481217EOE
CSB-EP481217EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Lipid-A-disaccharide synthase (lpxB) CSB-YP481218EOE
CSB-EP481218EOE
CSB-BP481218EOE
CSB-MP481218EOE
CSB-EP481218EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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