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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Phosphoheptose isomerase (gmhA) CSB-YP481219EOE
CSB-EP481219EOE
CSB-BP481219EOE
CSB-MP481219EOE
CSB-EP481219EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 GTPase Der (der) CSB-YP481220EOE
CSB-EP481220EOE
CSB-BP481220EOE
CSB-MP481220EOE
CSB-EP481220EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cytoskeleton protein rodZ (rodZ), partial CSB-YP481221EOE
CSB-EP481221EOE
CSB-BP481221EOE
CSB-MP481221EOE
CSB-EP481221EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-phenylpropionate/cinnamic acid dioxygenase subunit beta (hcaF) CSB-YP481222EOE
CSB-EP481222EOE
CSB-BP481222EOE
CSB-MP481222EOE
CSB-EP481222EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 LexA repressor (lexA) CSB-YP481223EOE
CSB-EP481223EOE
CSB-BP481223EOE
CSB-MP481223EOE
CSB-EP481223EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutamate 5-kinase (proB) CSB-YP481224EOE
CSB-EP481224EOE
CSB-BP481224EOE
CSB-MP481224EOE
CSB-EP481224EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosome maturation factor RimM (rimM) CSB-YP481225EOE
CSB-EP481225EOE
CSB-BP481225EOE
CSB-MP481225EOE
CSB-EP481225EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein nrdI (nrdI) CSB-YP481226EOE
CSB-EP481226EOE
CSB-BP481226EOE
CSB-MP481226EOE
CSB-EP481226EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Elongation factor P (efp) CSB-YP481227EOE
CSB-EP481227EOE
CSB-BP481227EOE
CSB-MP481227EOE
CSB-EP481227EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Fumarate reductase subunit C (frdC), partial CSB-YP481228EOE1
CSB-EP481228EOE1
CSB-BP481228EOE1
CSB-MP481228EOE1
CSB-EP481228EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein hfq (hfq) CSB-YP481229EOE
CSB-EP481229EOE
CSB-BP481229EOE
CSB-MP481229EOE
CSB-EP481229EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (ulaD) CSB-YP481230EOE
CSB-EP481230EOE
CSB-BP481230EOE
CSB-MP481230EOE
CSB-EP481230EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Anti-adapter protein iraP (iraP) CSB-YP481231EOE
CSB-EP481231EOE
CSB-BP481231EOE
CSB-MP481231EOE
CSB-EP481231EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Acyl carrier protein phosphodiesterase (acpH) CSB-YP481232EOE
CSB-EP481232EOE
CSB-BP481232EOE
CSB-MP481232EOE
CSB-EP481232EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP481233EOE
CSB-EP481233EOE
CSB-BP481233EOE
CSB-MP481233EOE
CSB-EP481233EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP481234EOE
CSB-EP481234EOE
CSB-BP481234EOE
CSB-MP481234EOE
CSB-EP481234EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP481235EOE
CSB-EP481235EOE
CSB-BP481235EOE
CSB-MP481235EOE
CSB-EP481235EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP481236EOE
CSB-EP481236EOE
CSB-BP481236EOE
CSB-MP481236EOE
CSB-EP481236EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Transcriptional repressor NrdR (nrdR) CSB-YP481237EOE
CSB-EP481237EOE
CSB-BP481237EOE
CSB-MP481237EOE
CSB-EP481237EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP481238EOE
CSB-EP481238EOE
CSB-BP481238EOE
CSB-MP481238EOE
CSB-EP481238EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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