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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse UPF0577 protein KIAA1324 (Kiaa1324), partial CSB-YP012250MO
CSB-EP012250MO
CSB-BP012250MO
CSB-MP012250MO
CSB-EP012250MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mediator of RNA polymerase II transcription subunit 12 (Med12), partial CSB-YP013643MO
CSB-EP013643MO
CSB-BP013643MO
CSB-MP013643MO
CSB-EP013643MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (Man1b1), partial CSB-YP013385MO1
CSB-EP013385MO1
CSB-BP013385MO1
CSB-MP013385MO1
CSB-EP013385MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Patatin-like phospholipase domain-containing protein 7 (Pnpla7), partial CSB-YP018286MO
CSB-EP018286MO
CSB-BP018286MO
CSB-MP018286MO
CSB-EP018286MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized coiled-coil domain-containing protein KIAA1984 (Kiaa1984) CSB-YP012300MO
CSB-EP012300MO
CSB-BP012300MO
CSB-MP012300MO
CSB-EP012300MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse MAP7 domain-containing protein 1 (Map7d1), partial CSB-YP013444MO
CSB-EP013444MO
CSB-BP013444MO
CSB-MP013444MO
CSB-EP013444MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein CXorf23 (CXorf23) CSB-YP006264HU
CSB-EP006264HU
CSB-BP006264HU
CSB-MP006264HU
CSB-EP006264HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein 804A (Znf804a), partial CSB-YP027054MO
CSB-EP027054MO
CSB-BP027054MO
CSB-MP027054MO
CSB-EP027054MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein Shroom2 (Shroom2), partial CSB-YP021282MO
CSB-EP021282MO
CSB-BP021282MO
CSB-MP021282MO
CSB-EP021282MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Centlein (Cntln), partial CSB-YP005685MO
CSB-EP005685MO
CSB-BP005685MO
CSB-MP005685MO
CSB-EP005685MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Caveolae-associated protein 4 (Cavin4) CSB-YP015239MO
CSB-EP015239MO
CSB-BP015239MO
CSB-MP015239MO
CSB-EP015239MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Targeting protein for Xklp2 (Tpx2) CSB-YP024139MO
CSB-EP024139MO
CSB-BP024139MO
CSB-MP024139MO
CSB-EP024139MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse BCL-6 corepressor-like protein 1 (Bcorl1), partial CSB-YP002636MO
CSB-EP002636MO
CSB-BP002636MO
CSB-MP002636MO
CSB-EP002636MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Peptidyl-prolyl cis-trans isomerase G (Ppig) CSB-YP018477MO
CSB-EP018477MO
CSB-BP018477MO
CSB-MP018477MO
CSB-EP018477MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leucine-rich repeat-containing G-protein coupled receptor 4 (Lgr4), partial CSB-YP012905MO
CSB-EP012905MO
CSB-BP012905MO
CSB-MP012905MO
CSB-EP012905MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein PAT1 homolog 2 (Patl2) CSB-YP017484MO
CSB-EP017484MO
CSB-BP017484MO
CSB-MP017484MO
CSB-EP017484MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ankyrin repeat domain-containing protein 16 (Ankrd16) CSB-YP001746MO
CSB-EP001746MO
CSB-BP001746MO
CSB-MP001746MO
CSB-EP001746MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Agrin (Agrn), partial CSB-YP001461MO
CSB-EP001461MO
CSB-BP001461MO
CSB-MP001461MO
CSB-EP001461MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Calcium-binding mitochondrial carrier protein SCaMC-2 (Slc25a25), partial CSB-YP021494MO1
CSB-EP021494MO1
CSB-BP021494MO1
CSB-MP021494MO1
CSB-EP021494MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial (Dhtkd1), partial CSB-YP006869MO
CSB-EP006869MO
CSB-BP006869MO
CSB-MP006869MO
CSB-EP006869MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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