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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Ubiquitin carboxyl-terminal hydrolase 4 (Usp4), partial CSB-YP025731RA
CSB-EP025731RA
CSB-BP025731RA
CSB-MP025731RA
CSB-EP025731RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse G-protein coupled receptor 161 (Gpr161), partial CSB-YP009774MO1
CSB-EP009774MO1
CSB-BP009774MO1
CSB-MP009774MO1
CSB-EP009774MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RNA polymerase I-specific transcription initiation factor RRN3 (Rrn3) CSB-YP020525MO
CSB-EP020525MO
CSB-BP020525MO
CSB-MP020525MO
CSB-EP020525MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Receptor-type tyrosine-protein phosphatase beta (Ptprb), partial CSB-YP019048MO
CSB-EP019048MO
CSB-BP019048MO
CSB-MP019048MO
CSB-EP019048MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Canalicular multispecific organic anion transporter 2 (Abcc3), partial CSB-YP001062MO
CSB-EP001062MO
CSB-BP001062MO
CSB-MP001062MO
CSB-EP001062MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein phosphatase 1 regulatory subunit 37 (Ppp1r37) CSB-YP013164RA
CSB-EP013164RA
CSB-BP013164RA
CSB-MP013164RA
CSB-EP013164RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat E3 ubiquitin-protein ligase RNF168 (Rnf168) CSB-YP019854RA
CSB-EP019854RA
CSB-BP019854RA
CSB-MP019854RA
CSB-EP019854RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Prostate and testis expressed protein 3 (Pate3) CSB-YP017481MO
CSB-EP017481MO
CSB-BP017481MO
CSB-MP017481MO
CSB-EP017481MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Low affinity cationic amino acid transporter 2 (SLC7A2), partial CSB-YP021714CH
CSB-EP021714CH
CSB-BP021714CH
CSB-MP021714CH
CSB-EP021714CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zinc finger protein 76 (Znf76) CSB-YP027017RA
CSB-EP027017RA
CSB-BP027017RA
CSB-MP027017RA
CSB-EP027017RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Rho guanine nucleotide exchange factor 2 (ARHGEF2), partial CSB-YP002050PI
CSB-EP002050PI
CSB-BP002050PI
CSB-MP002050PI
CSB-EP002050PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Eukaryotic translation initiation factor 5B (Eif5b), partial CSB-YP007581RA
CSB-EP007581RA
CSB-BP007581RA
CSB-MP007581RA
CSB-EP007581RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial (Oxct1) CSB-YP017306RA
CSB-EP017306RA
CSB-BP017306RA
CSB-MP017306RA
CSB-EP017306RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat NTF2-related export protein 2 (Nxt2) CSB-YP016232RA
CSB-EP016232RA
CSB-BP016232RA
CSB-MP016232RA
CSB-EP016232RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Protein delta homolog 2 (DLK2), partial CSB-YP006946PI1
CSB-EP006946PI1
CSB-BP006946PI1
CSB-MP006946PI1
CSB-EP006946PI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein 518A (Znf518a), partial CSB-YP026808MO
CSB-EP026808MO
CSB-BP026808MO
CSB-MP026808MO
CSB-EP026808MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein unc-13 homolog D (Unc13d), partial CSB-YP025624MO
CSB-EP025624MO
CSB-BP025624MO
CSB-MP025624MO
CSB-EP025624MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Histone acetyltransferase p300 (Ep300), partial CSB-YP007702MO
CSB-BP007702MO
CSB-MP007702MO
CSB-EP007702MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger and BTB domain-containing protein 42 (ZBTB42) CSB-YP026334HU
CSB-EP026334HU
CSB-BP026334HU
CSB-MP026334HU
CSB-EP026334HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat WAS protein family homolog 1 (Wash1) CSB-YP025972RA
CSB-EP025972RA
CSB-BP025972RA
CSB-MP025972RA
CSB-EP025972RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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