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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Leucine-rich repeat and fibronectin type-III domain-containing protein 5 (Lrfn5), partial CSB-YP013081RA1
CSB-EP013081RA1
CSB-BP013081RA1
CSB-MP013081RA1
CSB-EP013081RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Kelch-like protein 21 (Klhl21) CSB-YP012423RA
CSB-EP012423RA
CSB-BP012423RA
CSB-MP012423RA
CSB-EP012423RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cysteine and histidine-rich domain-containing protein 1 (Chordc1) CSB-YP005371RA
CSB-EP005371RA
CSB-BP005371RA
CSB-MP005371RA
CSB-EP005371RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein-glutamine gamma-glutamyltransferase E (Tgm3) CSB-YP023463RA
CSB-EP023463RA
CSB-BP023463RA
CSB-MP023463RA
CSB-EP023463RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ubinuclein-2 (Ubn2), partial CSB-YP025506RA
CSB-EP025506RA
CSB-BP025506RA
CSB-MP025506RA
CSB-EP025506RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tetratricopeptide repeat protein 19, mitochondrial (Ttc19) CSB-YP025211RA
CSB-EP025211RA
CSB-BP025211RA
CSB-MP025211RA
CSB-EP025211RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat E3 ubiquitin-protein ligase RNF152 (Rnf152), partial CSB-YP019848RA1
CSB-EP019848RA1
CSB-BP019848RA1
CSB-MP019848RA1
CSB-EP019848RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat GPN-loop GTPase 2 (Gpn2) CSB-YP009725RA
CSB-EP009725RA
CSB-BP009725RA
CSB-MP009725RA
CSB-EP009725RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Leucine-rich repeat transmembrane neuronal protein 2 (Lrrtm2), partial CSB-YP013188RA1
CSB-EP013188RA1
CSB-BP013188RA1
CSB-MP013188RA1
CSB-EP013188RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zinc finger FYVE domain-containing protein 26 (Zfyve26), partial CSB-YP026474RA
CSB-EP026474RA
CSB-BP026474RA
CSB-MP026474RA
CSB-EP026474RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 60S ribosomal protein L37a (Rpl37a) CSB-YP020267RA
CSB-EP020267RA
CSB-BP020267RA
CSB-MP020267RA
CSB-EP020267RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat F-box/LRR-repeat protein 15 (Fbxl15) CSB-YP008466RA
CSB-EP008466RA
CSB-BP008466RA
CSB-MP008466RA
CSB-EP008466RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ret finger protein-like 4A (Rfpl4a) CSB-YP019602RA
CSB-EP019602RA
CSB-BP019602RA
CSB-MP019602RA
CSB-EP019602RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat F-box only protein 3 (Fbxo3) CSB-YP008495RA
CSB-EP008495RA
CSB-BP008495RA
CSB-MP008495RA
CSB-EP008495RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Leucine-rich repeat and fibronectin type-III domain-containing protein 4 (Lrfn4), partial CSB-YP013080RA1
CSB-EP013080RA1
CSB-BP013080RA1
CSB-MP013080RA1
CSB-EP013080RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat C-type lectin domain family 9 member A (Clec9a), partial CSB-YP005542RA1
CSB-EP005542RA1
CSB-BP005542RA1
CSB-MP005542RA1
CSB-EP005542RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neuropilin and tolloid-like protein 2 (Neto2), partial CSB-YP015716RA1
CSB-EP015716RA1
CSB-BP015716RA1
CSB-MP015716RA1
CSB-EP015716RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative protein FAM157A (FAM157A) CSB-YP008099HU
CSB-EP008099HU
CSB-BP008099HU
CSB-MP008099HU
CSB-EP008099HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Selenoprotein W (SEPW1) CSB-YP021034CH
CSB-EP021034CH
CSB-BP021034CH
CSB-MP021034CH
CSB-EP021034CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine E3 ubiquitin-protein ligase TRIM56 (TRIM56), partial CSB-YP024494BO
CSB-EP024494BO
CSB-BP024494BO
CSB-MP024494BO
CSB-EP024494BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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