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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human PHD finger protein 1 (PHF1) CSB-YP017897HU
CSB-EP017897HU
CSB-BP017897HU
CSB-MP017897HU
CSB-EP017897HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Kallikrein-10 (KLK10) CSB-YP012447HU
CSB-EP012447HU
CSB-BP012447HU
CSB-MP012447HU
CSB-EP012447HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Embryonal Fyn-associated substrate (EFS) CSB-YP007470HU
CSB-EP007470HU
CSB-BP007470HU
CSB-MP007470HU
CSB-EP007470HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 264 (ZNF264) CSB-YP026622HU
CSB-EP026622HU
CSB-BP026622HU
CSB-MP026622HU
CSB-EP026622HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rho guanine nucleotide exchange factor 9 (ARHGEF9) CSB-YP002057HU
CSB-EP002057HU
CSB-BP002057HU
CSB-MP002057HU
CSB-EP002057HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitogen-activated protein kinase kinase kinase 7 (MAP3K7) CSB-YP013429HU
CSB-EP013429HU
CSB-BP013429HU
CSB-MP013429HU
CSB-EP013429HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) CSB-YP019736HU
CSB-BP019736HU
CSB-MP019736HU
CSB-EP019736HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human U4/U6 small nuclear ribonucleoprotein Prp3 (PRPF3) CSB-YP018763HU
CSB-EP018763HU
CSB-BP018763HU
CSB-MP018763HU
CSB-EP018763HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Synaptojanin-1 (SYNJ1), partial CSB-YP023016HU
CSB-EP023016HU
CSB-BP023016HU
CSB-MP023016HU
CSB-EP023016HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Trans-Golgi network integral membrane protein 2 (TGOLN2), partial CSB-YP023468HU1
CSB-EP023468HU1
CSB-BP023468HU1
CSB-MP023468HU1
CSB-EP023468HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (B3GNT1), partial CSB-YP002499HU1
CSB-EP002499HU1
CSB-BP002499HU1
CSB-MP002499HU1
CSB-EP002499HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mothers against decapentaplegic homolog 6 (SMAD6) CSB-YP021792HU
CSB-EP021792HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Epsilon-sarcoglycan (SGCE), partial CSB-YP021185HU1
CSB-EP021185HU1
CSB-BP021185HU1
CSB-MP021185HU1
CSB-EP021185HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Synaptotagmin-7 (SYT7), partial CSB-YP023043HU1
CSB-EP023043HU1
CSB-BP023043HU1
CSB-MP023043HU1
CSB-EP023043HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neuronal migration protein doublecortin (DCX) CSB-YP006576HU
CSB-BP006576HU
CSB-MP006576HU
CSB-EP006576HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Trafficking protein particle complex subunit 3 (TRAPPC3) CSB-YP024229HU
CSB-EP024229HU
CSB-BP024229HU
CSB-MP024229HU
CSB-EP024229HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytoplasmic protein NCK2 (NCK2) CSB-YP015531HU
CSB-BP015531HU
CSB-MP015531HU
CSB-EP015531HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (NDUFB3), partial CSB-YP015645HU1
CSB-EP015645HU1
CSB-BP015645HU1
CSB-MP015645HU1
CSB-EP015645HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human A-kinase anchor protein 7 isoforms alpha and beta (AKAP7) CSB-YP001529HU
CSB-EP001529HU
CSB-BP001529HU
CSB-MP001529HU
CSB-EP001529HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized methyltransferase WBSCR22 (WBSCR22) CSB-YP025985HU
CSB-EP025985HU
CSB-BP025985HU
CSB-MP025985HU
CSB-EP025985HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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